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本文旨在探讨小鼠黑色素瘤细胞及培养提取液对小鼠后肢缺血组织血管新生的影响,以及细胞趋化因子基质细胞衍生因子1(stromal cell derived factor1,SDF-1)及其特异性趋化因子受体(CXC chemokine receptor4,CXCR4)是否在这种影响中发挥作用。制作小鼠后肢缺血模型,在腹部皮下注射小鼠黑色素瘤细胞B16-F10或在缺血后肢肌肉注射细胞培养上清液,分别于7,14和21天行激光多普勒扫描(laser Doppler perfusion imaging,LDPI)评估后肢缺血组织血流灌注情况,21天后流式细胞术分析小鼠骨髓细胞(bone marrow cells,BMCs)中的CXCR4表达,碱性磷酸酶染色后组织学分析缺血后肢血管新生情况。结果显示,与对照组相比,肿瘤细胞和细胞培养液均可使BMCs中CXCR4阳性表达细胞数增加(P<0.05),小鼠缺血后肢血流灌注情况明显改善(P<0.05),小鼠缺血后肢肌肉毛细血管密度显著增加(P<0.05)。以上结果提示,黑色素瘤细胞及细胞培养液可以促进BMCs中CXCR4的表达,通过SDF-1/CXCR4途径促进缺血组织的血管新生。
This article aims to investigate the effects of mouse melanoma cells and culture extracts on the angiogenesis of mouse hindlimb ischemic tissue, as well as the effects of the cell chemotactic factor stromal cell derived factor 1 (SDF-1) Whether CXC chemokine receptor 4 (CXCR4) plays a role in this effect. Hindlimb ischemia models were made in mice. Mice melanoma B16-F10 cells were subcutaneously injected into the abdomen or intramuscularly injected into the culture supernatant of ischemic hindlimbs. Laser Doppler scanning was performed on days 7, 14 and 21 perfusion imaging (LDPI) was used to evaluate the perfusion of hind limb ischemic tissue. The expression of CXCR4 in mouse bone marrow cells (BMCs) was analyzed by flow cytometry 21 days later. Angiogenesis. The results showed that compared with the control group, the number of CXCR4 positive cells in BMCs increased (P <0.05) and the perfusion of ischemic hindlimb in mice significantly improved (P <0.05) compared with the control group The hyaline capillary density of hind limbs increased significantly (P <0.05). The above results suggest that melanoma cells and cell culture medium can promote the expression of CXCR4 in BMCs and promote the angiogenesis of ischemic tissue through the SDF-1 / CXCR4 pathway.