论文部分内容阅读
Aeromonas hydrophila (A. hydrophila) 4AK4 produced poly(3 hydroxybutyrate co 3 hydroxyhexanoate) (PHBHHx) with an almost constant 3 hydroxyhexanoate (3HHx) content of 10%15% from lauric acid and/or soybean oil. Both A. hydrophila 4AK4 and recombinant Escherichia coli (E. coli) JMU193 (pBH32) produced PHBHHx with controllable 3HHx content when fed lauric acid and another co substrate. With glucose or gluconate as the co substrate, the 3HHx content in the copolyester produced by A. hydrophila 4AK4 was reduced slightly from 12% to 9%. However, the 3HHx content in the copolyester produced by E. coli JMU193 (pBH32) was significantly reduced from 9% to 2% with fructose as the co substrate. These results show that regulation of 3HHx content in PHBHHx can be achieved using genetically engineered E. coli.
Both A. hydrophila 4AK4 produced poly (3 hydroxybutyrate co 3 hydroxyhexanoate) (PHBHHx) with an almost constant 3 hydroxyhexanoate (3HHx) content of 10% 15% from lauric acid and / or soybean oil. Both A. hydrophila 4AK4 and recombinant Escherichia coli (E. coli) JMU193 (pBH32) produced PHBHHx with controllable 3HHx content when fed lauric acid and another co substrate. With glucose or gluconate as the co substrate, the 3HHx content in the copolyester produced by A. hydrophila 4AK4 was reduced slightly from 12% to 9%. However, the 3HHx content in the copolyester produced by E. coli JMU193 (pBH32) was significantly reduced from 9% to 2% with fructose as the co substrate. These results show that regulation of 3HHx content in PHBHHx can be using using genetically engineered E. coli.