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目的分析评估恒河猴遗传多样性水平,为建立标准化的恒河猴监测方法提供基础资料。方法利用19个微卫星DNA标记对恒河猴遗传多样性进行分析,经基因组DNA提取、PCR扩增、聚丙烯酰胺凝胶电泳等,用POPGENE 1.32软件及Excel进行统计分析。结果 19个微卫星座位均呈现出了高度多态性,共检测到了等位基因164个,各座位的观察等位基因数在6～11个之间,平均为8.6316个;各基因座位的有效等位基因数在3.5727～8.9416个之间,平均为6.0709个;各微卫星座位的观察杂合度在0.2560～0.6364之间,群体平均值为0.4309;期望杂合度在0.7230～0.9010之间,群体平均值为0.8270;多态信息含量在0.6771～0.8874之间,群体平均值为0.7979;香隆信息指数在1.4249～2.2662之间,群体平均值为1.8901;本研究检测的19个微卫星座位均偏离Hardy-Weinberg平衡(P<0.01)。结论所研究的恒河猴群体遗传多态性比较丰富,本实验为今后建立恒河猴质量监测方法提供理论依据。
Objective To analyze and evaluate the genetic diversity of rhesus monkeys and provide basic information for establishing a standardized monitoring method for rhesus monkeys. Methods Nineteen microsatellite DNA markers were used to analyze the genetic diversity of rhesus monkeys. Genomic DNA extraction, PCR amplification and polyacrylamide gel electrophoresis were used to analyze the genetic diversity of rhesus monkeys. POPGENE 1.32 software and Excel were used for statistical analysis. Results All 19 microsatellite loci showed high polymorphism. A total of 164 alleles were detected. The number of alleles observed in each locus ranged from 6 to 11 with an average of 8.6316. The loci were effective The average number of alleles was between 3.5727 and 8.9416, with an average of 6.0709. The observed heterozygosities of microsatellite loci ranged from 0.2560 to 0.6364, and the population average was 0.4309. The expected heterozygosity ranged from 0.7230 to 0.9010. The population mean Value of 0.8270; polymorphism information content between 0.6771 ~ 0.8874, population average of 0.7979; Aroma information index between 1.4249 ~ 2.2662, population average of 1.8901; 19 microsatellite loci were deviated from Hardy -Weinberg equilibrium (P <0.01). Conclusion The Rhesus macaque population genetic polymorphism is rich. This study provides a theoretical basis for establishing the quality monitoring method of rhesus monkeys in the future.