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目的 :在真核细胞中表达人lrg基因。 方法 :构建野生型人lrg的真核表达载体 ,体外转染肝癌细胞系HepG2 ,并进行稳定筛选。用WesternBlot、SABC -FITC进行人lrg基因表达的鉴定。 结果 :成功构建了人lrg的真核表达载体 pcDNA3.1( + ) /hlrg ,WesternBlot和SABC -FITC等实验表明该基因在HepG2中进行了表达。结论 :在HepG2中稳定表达了 pcDNA3.1( + ) /hlrg ,为深入探讨人lrg基因的功能奠定了基础。
Purpose: To express human lrg gene in eukaryotic cells. Methods: The eukaryotic expression vector of wild-type human lrg was constructed and transfected into HepG2 hepatoma cell line in vitro and stably screened. Identification of human lrg gene expression using WesternBlot, SABC-FITC. Results: The eukaryotic expression vector pcDNA3.1 (+) / hlrg of human lr was successfully constructed, and Western Blot and SABC-FITC experiments showed that the gene was expressed in HepG2. Conclusion: pcDNA3.1 (+) / hlrg was stably expressed in HepG2, which lays a foundation for further exploration of human lrg gene function.