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油菜菌核病是危害油菜生产的主要病害,多菌灵作为防治该病害的有效杀菌剂在很多地区已产生抗性.川渝地区是我国油菜的重要产区,油菜菌核病发生普遍,但该地区病原菌对多菌灵抗性研究未见报道.该文开展川渝地区油菜菌核病对多菌灵敏感性基线、抗性监测及抗性分子机理的研究,采用菌丝生长速率法测定了106个野生菌株对多菌灵的敏感性,结果表明病原菌EC50值在0.009 12~0.182 7μg/mL之间,不同敏感性的菌株频率呈近连续的单峰曲线分布,平均EC50值为(0.067 25±0.034 06)μg/mL;以5.0μg/mL作为田间抗性菌株的区分剂量,检测了2013年采自该地区10个区、县、市585株病原菌对多菌灵的抗性,结果表明绝大多数为敏感菌株,占99.49%,仅发现3株田间抗性菌株,占0.51%;3株抗性菌株的抗性倍数达2 085倍以上,均为高抗菌株;以引物B1-1/B3-1PCR扩增抗性菌株的β-微管蛋白基因,测序并与标准菌株1 980比对,结果发现3株抗性菌株的198位氨基酸均由Glu变为Ala.
Sclerotinia sclerotiorum is the main disease that endanger the production of rapeseed, and carbendazim, as an effective bactericide to control the disease, has developed resistance in many areas. Chuan-Yu region is an important producing region of rapeseed in China. Sclerotinia sclerotiorum is common but The study on the resistance of carbendazim to pathogens in this area has not been reported.In this paper, the sensitivity of carbendazim to the baseline, resistance monitoring and molecular mechanism of resistance in rapeseed sclerotinia in Sichuan and Chongqing was studied. The mycelial growth rate The results showed that the EC50 values of pathogenic bacteria ranged from 0.009 12 to 0.182 7 μg / mL. The frequencies of different susceptible strains showed nearly continuous unimodal distribution with the mean EC50 value of (0.067 25 ± 0.034 06) μg / mL. The resistance to carbendazim in 585 strains of pathogens collected from 10 districts, counties and cities in the region in 2013 was tested at a dose of 5.0 μg / mL as field-resistant strains. The results showed that The majority of them were sensitive strains, accounting for 99.49%, only 3 strains of field-resistant were found, accounting for 0.51%. The resistance of 3 strains was over 2 085 times, which were all highly antibacterial strains. / B3-1 PCR The beta-tubulin gene of the resistant strain was amplified, sequenced and aligned with the standard 1980 strain comparison, the amino acid found 198 3 becomes resistant strains by Glu Ala.