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目的:通过肠道门诊标本检出的聚集性大肠埃希菌,研究其相关致病因子的毒力基因。方法:使用生物膜形成试验筛检聚集性大肠埃希菌,获得菌株后,进行转译激活因子基因,CVD432质粒基因,聚集性大肠埃希菌Ⅰ型鞭毛基因、Ⅱ型鞭毛基因、Ⅲ型鞭毛基因、Ⅳ菌毛基因,质粒编码毒素基因,聚集性大肠埃希菌耐热性肠毒素基因等各种基因检测,研究不同基因的分布和生物膜实验的相关性。结果:可以通过生物膜形成试验筛检聚集性大肠埃希菌,并根据毒力基因分型。结论:生物膜形成实验作为利用生物学特性筛检聚集性大肠埃希菌有一定的应用价值,聚集性大肠埃希菌的生物膜的形成与其各类毒力基因有不同程度的相关性。
OBJECTIVE: To study the virulence genes of enterohalobacter jejuni isolated from intestinal gut samples. Methods: The biofilm formation assay was used to screen for the aggregated Escherichia coli. After the strain was obtained, the gene encoding translation activator, CVD432, conglomerate type Ⅰ flagella, type Ⅱ flagella and type Ⅲ flagella , Ⅳ pili gene, plasmid encoding toxin gene, E. coli heat-labile enterotoxin gene and other genes were tested to study the distribution of different genes and the relevance of biofilm experiments. Results: E. coli can be screened by biofilm formation assays and genotyped by virulence. CONCLUSION: Biofilm formation assay has certain value as a biomarker for the screening of E.coli with biological characteristics. The biofilm formation of E.coli in aggregation is associated with various degrees of virulence genes.