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目的:探讨新型脂联素受体T-钙黏蛋白(T-cadherin)在非酒精性脂肪性肝病SD大鼠肝及血液中的表达及其与肝组织病理改变的关系。方法:将60只SD大鼠随机均分为2组:模型组(高脂饲料喂养)及正常组(普通饲料喂养)。分别于第0,6,8,12及16周各组处死6只SD大鼠。监测大鼠体质量和肝指数变化,利用肝组织病理切片观察肝脂肪沉积情况,进行NAFLD活动度积分(NAFLD activity score,NAS)。以RT-PCR法检测肝及血液T-cadherin mRNA表达及其动态变化,免疫组织化学检测肝T-cadherin蛋白表达及其动态变化。结果:模型组大鼠肝及血液中T-cadherinmRNA表达均逐步上升,在造模6周后即与正常组比较差异具有统计学意义(P<0.01)。肝T-cadherinmRNA水平与肝指数(r=0.900,P<0.01)、肝NAS(r=0.933,P<0.01)均显著正相关。血液中存在可溶性的T-cadherin成分,其水平与肝指数(r=0.805,P<0.01)、肝NAS评分(r=0.889,P<0.01)亦呈正相关。模型组大鼠肝中T-cadherin蛋白表达逐步上升,在造模6周后即与正常组比较差异具有统计学意义(P<0.05)。肝T-cadherin蛋白水平与肝指数(r=0.879,P<0.01)、肝脏NAS评分(r=0.912,P<0.01)均显著正相关。结论:非酒精性脂肪性肝病大鼠肝及血液中可溶性T-cadherin mRNA及蛋白的表达与肝脂肪性炎症程度呈正相关,提示T-cadherin与非酒精性脂肪性肝病的发病密切相关。
Objective: To investigate the expression of T-cadherin, a novel adiponectin receptor, in the liver and blood of SD rats with non-alcoholic fatty liver disease and its relationship with pathological changes of liver tissue. Methods: Sixty SD rats were randomly divided into two groups: model group (fed with high fat diet) and normal group (fed with normal diet). Six SD rats were sacrificed at 0, 6, 8, 12 and 16 weeks respectively. The body weight and liver index of rats were monitored. The hepatic fat deposition was observed by pathological sections of liver and NAFLD activity score (NAS) was calculated. The expression of T-cadherin mRNA and its dynamic changes in liver and blood were detected by RT-PCR. The expression of T-cadherin in liver and its dynamic changes were detected by immunohistochemistry. Results: The expression of T-cadherin mRNA in the liver and blood of the model group increased gradually, and there was significant difference between the model group and the normal group 6 weeks after modeling (P <0.01). Liver T-cadherin mRNA levels were significantly and positively correlated with liver index (r = 0.900, P <0.01) and liver NAS (r = 0.933, P <0.01). The level of soluble T-cadherin in blood was positively correlated with liver index (r = 0.805, P <0.01) and liver NAS score (r = 0.889, P <0.01). The expression of T-cadherin protein in the liver of model group increased gradually, and there was significant difference between the model group and the normal group 6 weeks after modeling (P <0.05). Liver T-cadherin protein levels were significantly and positively correlated with liver index (r = 0.879, P <0.01) and hepatic NAS score (r = 0.912, P <0.01). Conclusion: The expression of soluble T-cadherin mRNA and protein in the liver and blood of non-alcoholic fatty liver disease is positively correlated with the degree of hepatic steatosis, suggesting that T-cadherin is closely related to the pathogenesis of nonalcoholic fatty liver disease.