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目的探讨白藜芦醇对日本血吸虫病纤维化的作用,并分析其对Th1和Th2应答的影响。方法 45只C57BL/6小鼠感染日本血吸虫3周后,随机分为A、B、C 3组,A组为感染组,B组为灌胃白藜芦醇治疗组,C组为灌胃吡喹酮治疗组,另取15只正常C57BL/6小鼠为健康对照D组。在感染第13周,取小鼠的肝脏,应用天狼猩红染色观察肝脏纤维化程度,并检测肝脏中IL-13、IFN-γ和TGF-β mRNA的表达情况。另取脾脏淋巴细胞,采用流式细胞术检测Th1和Th2细胞占总T淋巴细胞的比例。结果感染第13周,与A组相比,B组和C组小鼠肝脏纤维化程度明显减轻(P<0.01),B组小鼠的Th1细胞比例明显增高(P<0.05),Th2细胞比例明显降低(P<0.01),B组小鼠外周血清中的抗可溶性成虫抗原(SWA)的IgG2a明显增多(P<0.05),而抗可溶性虫卵抗原(SEA)的IgG1明显减少(P<0.01),B组小鼠肝脏中IFN-γ mRNA的表达量明显增高(P<0.05),而IL-13、TGF-β mRNA的表达水平明显降低(P<0.01)。结论白藜芦醇通过增强日本血吸虫感染小鼠的Th1应答,降低Th2应答,明显抑制日本血吸虫感染小鼠肝脏纤维化程度。
Objective To investigate the effect of resveratrol on the fibrosis of Schistosoma japonicum and analyze its effect on Th1 and Th2 responses. Methods 45 C57BL / 6 mice were infected with Schistosoma japonicum for 3 weeks and were randomly divided into A, B and C groups. A group was infection group, B group was treated with resveratrol, The other 15 normal C57BL / 6 mice served as healthy control group D. At the 13th week of infection, the hepatic fibrosis was observed by Sirius red staining and the expression of IL-13, IFN-γ and TGF-β mRNA in the liver was detected. Another spleen lymphocytes, the use of flow cytometry Th1 and Th2 cells in total T lymphocyte ratio. Results At the 13th week of infection, compared with group A, the degree of liver fibrosis in group B and group C was significantly reduced (P <0.01), and the proportion of Th1 cells in group B was significantly increased (P <0.05). The proportion of Th2 cells (P <0.01). The IgG2a level of anti-soluble adult antigen (SWA) in peripheral blood of group B was significantly increased (P <0.05), while the IgG1 of anti-soluble antigen was significantly decreased ), The expression of IFN-γ mRNA in liver of group B was significantly increased (P <0.05), while the expression of IL-13 and TGF-β mRNA was significantly decreased (P <0.01). Conclusion Resveratrol can reduce the Th2 response in mice infected with Schistosoma japonicum, and significantly inhibit the hepatic fibrosis in mice infected with Schistosoma japonicum.