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目的建立鲑鱼降钙素磷脂复合物的含量测定方法。方法采用HPLC法,Vydac 218TP54 C18色谱柱(250 mm×4.6mm,5μm,300),流动相为0.02 mol·L-1四甲基氢氧化铵缓冲液(磷酸调pH2.5)-乙腈(A:9∶1,B:2∶3),梯度洗脱程序为:0~21 min、65%~43%A,21.01~24 min、65%A,流速1.0 mL·min-1,检测波长220 nm,柱温40℃,进样量20μL。结果鲑鱼降钙素检测浓度的线性范围为4.49-89.87μg·mL-1(r=0.9995),平均回收率为99.56%(RSD=1.48%)。结论所用方法准确可靠、重复性好,可用于鲑鱼降钙素磷脂复合物的含量测定。
Objective To establish a method for the determination of salmon calcitonin phospholipid complex. Methods HPLC was performed on a Vydac 218TP54 C18 column (250 mm × 4.6 mm, 5 μm, 300 μm) with a mobile phase of 0.02 mol·L -1 tetramethylammonium hydroxide (adjusted to pH 2.5 with phosphoric acid) -acetonitrile A: 9: 1, B: 2: 3). The gradient elution program was: 0-21 min, 65% -43% A, 21.01-24 min, 65% A, 220 nm, column temperature 40 ℃, injection volume 20μL. Results The linear range of salmon calcitonin was 4.49-89.87μg · mL-1 (r = 0.9995). The average recovery was 99.56% (RSD = 1.48%). Conclusion The method is accurate and reproducible, and can be used for the determination of salmon calcitonin phospholipid complex.