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目的:研究高效液相色谱法检测早期胃癌中胃液固有荧光光谱的诊断价值.方法:选择2004-08/2008-03在北京大学第三医院胃镜检查患者的胃液,采用MonoQ离子交换高效液相色谱分析方法检测胃液固有荧光,比较早期胃癌与胃良性疾病间的荧光峰差异并进行统计分析.结果:胃液在MonoQ离子交换高效液相色谱条件下于保留时间30-32min之间规律出现一个强度最大的荧光峰,早期胃癌组均值明显高于胃良性病变组(P<0.001).以荧光强度0.12V为判别界值,诊断早期胃癌的敏感度是91.2%、特异度是87.8%.结论:高效液相色谱法检测胃液固有荧光用于诊断早期胃癌具有较高的敏感性和特异性.
OBJECTIVE: To study the diagnostic value of high performance liquid chromatography (HPLC) in the detection of gastric intrinsic fluorescence spectra in early stage gastric cancer.Methods: The gastric juice of patients with gastroscopy in Peking University Third Hospital from August 2004 to March 2008 was analyzed by MonoQ ion exchange high performance liquid chromatography Analyze the intrinsic fluorescence of gastric juice and compare the difference of fluorescence peak between early gastric cancer and gastric benign disease and make statistical analysis.Results: The gastric juice showed the highest intensity at the retention time of 30-32min with MonoQ ion-exchange high performance liquid chromatography (P <0.001) .According to the fluorescence intensity of 0.12V, the sensitivity and specificity were 91.2% and 87.8% respectively.Conclusion: High efficiency Liquid chromatography detection of gastric intrinsic fluorescence for the diagnosis of early gastric cancer has a high sensitivity and specificity.