论文部分内容阅读
目的 探讨不同细胞对砷的基因毒性反应及其机理 ;探讨快速、敏感及经济的细胞基因毒性检测方法。方法 应用单细胞凝胶电泳 (Single cell gel electrophoresis,SCGE)或彗星试验 (com et assay)比较研究。结果 细胞 DNA损伤与砷处理量间呈显著的剂量反应关系和时效关系。受试细胞对砷的基因毒性反应的敏感性强弱顺序为 :PMA- HL 6 0 >DMSO- HL 6 0 >HL 6 0 >淋巴细胞。人工计数彗星尾分级频数与计算机自动测量彗星尾 DNA泳动量的结果完全一致 ,用非参数 Mann- Whitney多变量检验分析彗星尾分级频数的显著性与参数Tukey多变量检验分析量彗星尾 DNA泳动量的显著性完全相同 ,而且不同浓度的砷与 5级彗星尾频数的相关性比其与阳性彗星尾总数间的更为显著。结论 微量砷即可引起细胞不同程度的 DNA损伤 ,不同细胞亚群对砷的基因毒性反应不同。 SCGE是一个简便、快速、敏感及经济的细胞基因毒性检测方法 ,可应用于各级水平的环境与人类疾病 (砷污染 )监测与研究
OBJECTIVE: To investigate the genotoxic response of arsenic to different cells and its mechanism, and to explore a rapid, sensitive and economical method of detection of genotoxicity in cells. Methods Single cell gel electrophoresis (SCGE) or com et assay was used to compare the results. Results There was a dose-response and time-dependent relationship between DNA damage and arsenic content. The sensitivity and the order of the tested cells to arsenic genotoxicity were as follows: PMA-HL 6 0> DMSO-HL 6 0> HL 6 0> lymphocytes. The counting frequency of the tail of the comet was manually calculated and the results of computer automatic measurement of the tail DNA migration of the comet were completely consistent. The significance and parameters of the tailing frequency of the comet tail were analyzed by the non-parametric Mann-Whitney test. The significance of the same, and different concentrations of arsenic and grade 5 comet tail frequency correlation with the total number of positive comets than the total between the more significant. Conclusion Arsenic trioxide can cause cell DNA damage to varying degrees, different cell subgroups of genotoxic response to arsenic different. SCGE is a simple, rapid, sensitive and economical method of detection of cellular genotoxicity and can be applied to the monitoring and research of environmental and human diseases (arsenic pollution) at all levels