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目的:通过观察百合地黄汤对创伤后应激障碍(PTSD)大鼠行为学改变及海马5-HT水平的影响,探讨百合地黄汤对PTSD的干预作用。方法:将60只SPF级雄性SD大鼠,随机分为正常组、模型组、对照组、低剂量组、中剂量组和高剂量组,每组10只。空白组给予常规无干扰喂养2周,余5组采用SPS法造模后,分为百合地黄汤低剂量组[2.5 g/(kg·d)]、百合地黄汤中剂量组[5.0 g/(kg·d)]、百合地黄汤高剂量组[10 g/(kg·d)]、氟西汀对照组[2.4 mg/(kg·d)],按[20 mL/(kg·d)]、分二次灌胃,模型组予等量生理盐水。2周后采用高架十字迷宫(EPM)进行行为学评定,并用ELISA法检测大鼠海马5-HT水平。结果:模型组大鼠进入开臂次数、停留时间、海马组织5-HT水平均明显低于正常组;通过不同剂量百合地黄汤及氟西汀治疗后模型大鼠进入开臂次数、停留时间、海马5-HT水平明显提高,且高剂量百合地黄汤组明显优于氟西汀对照组(P<0.05)。结论:百合地黄汤对PTSD大鼠具有较好的干预治疗作用,上调海马5-HT水平可能是机制之一。
Objective: To observe the effects of Lily Rehmanniae Decoction on the behavioral changes and 5-HT level in hippocampus of rats with post traumatic stress disorder (PTSD) and to explore the intervention effect of Lily Dihuang Decoction on PTSD. Methods: Sixty SPF male SD rats were randomly divided into normal group, model group, control group, low dose group, middle dose group and high dose group, with 10 rats in each group. The blank group was given routine non-interference feeding for 2 weeks, and the other 5 groups were divided into the low dose group of Lily Rehmanniae Decoction [2.5 g / (kg · d)] and the middle dose Lily Rehmanniae Group [5.0 g / ( kg · d)], high dosage group of Lily Rehmanniae Decoction [10 g / (kg · d)] and fluoxetine control group (2.4 mg / (kg · d) , Divided into two gavages, the model group to the same amount of saline. Behavioral assessment was performed after 2 weeks using elevated plus maze (EPM), and 5-HT level in hippocampus was detected by ELISA. Results: The number of open arms, the residence time and the level of 5-HT in hippocampus of rats in model group were significantly lower than those in normal rats. The number of open arms, dwell time, Hippocampal 5-HT levels were significantly increased, and high-dose Lily Rehmanniae group was significantly better than fluoxetine control group (P <0.05). Conclusion: Lily Rehmannia Decoction has a good therapeutic effect on PTSD rats. Upregulation of 5-HT in hippocampus may be one of the mechanisms.