论文部分内容阅读
实验在１７只麻醉、自主呼吸的ＳＤ大鼠上进行，用多管微电极在旁巨细胞外侧核尾侧半（ｃＰＧＣＬ）观察了微电泳乙酰胆碱（ＡＣｈ）及其桔抗剂Ａｔｒｏｐｉｎｅ（阿托品）对神经元自发放电的效应及Ａｔｒｏｐｉｎｅ对ＡＣｈ效应的影响。ＡＣｈ可引起大多数被测神经元（７９．６％）兴奋，少数（２０．４％）无反应；Ａｔｒｏｐｉｎｅ可引起神经元兴奋（占被测神经元数的８％）、抑制（占４８％）或无反应（占４４％）。ＡＣｈ的兴奋效应和Ａｔｒｏｐｉｎｅ的抑制效应呈量效依赖关系。Ａｔｒｏｐｉｎｅ可部分或完全阻断大多数被测试神经元（８０．３％）对ＡＣｈ的兴奋反应。本结果提示ＰＧＣＬ区可能存在着起递质作用的内源性ＡＣｈ，某些神经元存在着Ｍ受体
The experiments were performed in 17 anesthetized and spontaneously breathing SD rats. Microelectrophoresis of acetylcholine (ACh) and its antitumor agent Atropine were observed with multi-tube microelectrodes on the caudallateral giant cell nucleus caudalis half (cPGCL) Effect of spontaneous firing on neurons and the effect of Atropine on ACh effect. ACh can cause most of the measured neurons (79.6%) excited, a few (20.4%) did not respond; Atropine can cause neuronal excitement (accounting for 8% of the measured neurons), inhibition ) Or no response (44%). The excitatory effect of ACh and the inhibitory effect of Atropine showed a dose-dependent relationship. Atropine partially or completely blocked the activation of ACh by most tested neurons (80.3%). Our results suggest that there may be endogenous ACh acting as a neurotransmitter in the PGCL region and M receptors in some neurons