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为了探讨板栗愈伤组织对栗疫病的抗性机制,采用栗疫菌Cp-毒素处理板栗愈伤组织,分析了毒素对不同抗性的板栗品种愈伤组织病程相关蛋白、植物保卫素及游离脯氨酸含量的影响。研究表明:在较低浓度毒素处理范围内(5~100μg/m L),板栗愈伤组织内的游离脯氨酸含量、植物保卫素含量、几丁质酶和β-1,3-葡聚糖酶活性与Cp-毒素有关,抗病品种酶活性升幅大于感病品种;当毒素质量浓度高于100μg/m L后,植物保卫素含量以及几丁质酶和β-1,3-葡聚糖酶活性下降,感病品种降幅大于抗病品种,而游离脯氨酸含量在品种间变化不大。说明在毒素低浓度处理下不同品种抗性物质含量都有所增加,证明了几丁质酶和β-1,3-葡聚糖酶、植物保卫素是板栗愈伤组织抵抗病原菌毒素的内在机制,而游离脯氨酸含量变化不是引起品种抗性差异的重要因子。
In order to investigate the resistance mechanism of chestnut callus to chestnut blight, chestnut callus was treated with Chestnut blight Cp-toxin, and the effects of toxins on callus histopathological proteins, plant preservatives, Amino acid content. The results showed that the content of free proline, the content of phytoalexin, the content of chitinase and β-1,3-glucuronidase in chestnut callus in the range of 5 ~ 100μg / m L, The activity of carbohydrase was related to Cp-toxin, and the increase of enzyme activity of resistant varieties was greater than that of susceptible varieties. When the concentration of toxin was higher than 100μg / m L, the content of phytoalexin and the activities of chitinase and β- The activity of carbohydrase decreased, the decline of susceptible varieties was greater than that of resistant varieties, while the content of free proline changed little among cultivars. The results showed that the content of resistant species increased with the low concentration of toxins, which proved that chitinase and β-1, 3-glucanase and phytoalexin were the intrinsic mechanisms of resistance of chestnut callus to pathogenic toxins , While the change of free proline content was not the important factor that caused the difference of resistance of varieties.