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目的检测乳腺癌及乳腺良性病变患者外周血中人乳腺珠蛋白(human mammary gland globin,h MAM)m RNA的表达,为乳腺癌分子标记选择提供理论依据。方法选取2011年6月至2012年10月期间笔者所在医院收治的乳腺癌患者78例以及乳腺良性病变患者30例(乳腺增生症15例,乳腺纤维腺瘤15例)作为研究对象,应用聚合酶链反应(PCR)技术检测其外周血中h MAM m RNA的表达,并分析其与患者年龄、肿瘤大小、病理学类型、肿瘤分期、腋窝淋巴结转移以及ER、PR和HER-2表达的关系。结果乳腺增生及乳腺纤维腺瘤患者外周血中均未检出h MAM m RNA表达,乳腺癌患者外周血中h MAM m RNA表达阳性率为48.72%(38/78)其差异有统计学意义(χ2=12.357,P=0.000)。乳腺癌患者外周血中h MAM m RNA的表达与患者年龄、肿瘤大小、病理学类型以及ER、PR和HER-2表达无关(P>0.05),但与肿瘤的临床分期(Z=-2.214,P=0.027)和淋巴结转移相关(Z=-2.754,P=0.006)。结论外周血中检出的h MAM m RNA表达是诊断乳腺癌相对特异性的标志,外周血中h MAM m RNA的表达可能与乳腺癌患者的预后相关。
Objective To detect the expression of human mammary gland globin (h MAM) m RNA in peripheral blood of patients with breast cancer and benign breast lesions, and to provide a theoretical basis for molecular marker selection of breast cancer. Methods From June 2011 to October 2012, 78 cases of breast cancer and 30 cases of benign breast lesions (15 cases of hyperplasia of mammary glands and 15 cases of fibroadenoma of the breast) were treated in our hospital from June 2011 to October 2012. Polymerase The expression of hMAM m RNA in peripheral blood was detected by polymerase chain reaction (PCR), and its relationship with age, tumor size, pathological type, tumor stage, axillary lymph node metastasis and ER, PR and HER-2 expression were analyzed. Results The expression of h MAM m RNA was not detected in peripheral blood of patients with breast hyperplasia and breast fibroadenoma. The positive rate of h MAM m RNA expression in peripheral blood of breast cancer patients was 48.72% (38/78), with significant difference ( χ2 = 12.357, P = 0.000). The expression of h MAM m RNA in peripheral blood of patients with breast cancer was not related to the age, tumor size, pathological type, ER, PR and HER-2 expression (P> 0.05), but correlated with the clinical stage (Z = -2.214, P = 0.027) and lymph node metastasis (Z = -2.754, P = 0.006). Conclusions The expression of h MAM m RNA in peripheral blood is a specific marker for diagnosis of breast cancer. The expression of h MAM m RNA in peripheral blood may be related to the prognosis of patients with breast cancer.