论文部分内容阅读
目的探讨高原环境下人体外周血红细胞形态与高原反应的关系,为高原反应的预防和治疗提供实验依据。方法设急进高原组:世居海拔约500 m平原地区的受试者25名,分别于其急进高原前后采集外周血血样各5m L/(人)份;久居高原组:受试者14名,居住在海拔约3 600 m地区>10年,包括世居高原的藏族人7名、居住高原10-15年的汉族人7名,采集其外周血血样5 m L/(人)份;调查2组的高原反应发生情况,扫描电镜观察2组受试者红细胞形态,统计分析2组受试者不同形态红细胞数量。结果在海拔约3 200及3 600 m高原地区,急进高原组均出现不同程度的高原反应,久居高原组则无高原反应情况发生。正常形态的外周血红细胞数量(个/份):急进高原组急进高原前后分别为471.43±1.35(海拔约500 m)、263.31±6.16(海拔约3 200 m)、256.29±13.03(海拔约3 600m)(P<0.01),且畸形红细胞数量明显增加;久居高原组为368.57±11.26,明显高于急进高原组(P<0.01),且畸形红细胞数量明显低于急进高原组。久居高原组中藏族和汉族人群的正常形态的外周血红细胞数量相近(P>0.05)。结论正常健康成年人急进高原后,外周血红细胞的畸形化改变可能是高原反应发生的机理之一。
Objective To explore the relationship between the peripheral erythrocyte morphology and altitude sickness in the plateau environment and to provide experimental evidence for the prevention and treatment of altitude sickness. Methods The rapid plateau group was established. Twenty-five subjects living in an area of about 500 m above sea level were collected, and 5 m L / (human) samples of peripheral blood were collected before and after their rapid plateau. In the long-term plateau group, 14 subjects, Living in an area of about 3 600 m above sea level for more than 10 years, including 7 Tibetans living in the plateau, 7 Han Chinese living in the plateau for 10-15 years and collecting 5 m L / (person) of peripheral blood samples; Investigation 2 Group of plateau reaction occurred, the scanning electron microscope observation of two groups of subjects erythrocyte morphology, statistical analysis of two groups of subjects with different forms of red blood cell number. Results In the plateau areas of about 3 200 and 3 600 m above sea level, all the plateau reactions experienced different degrees of plateau in the plateau, and no plateau occurred in the plateau for a long time. The number of normal peripheral blood red blood cells (a / copies): before and after the plateau group was 471.43 ± 1.35 (elevation about 500 m), 263.31 ± 6.16 (elevation about 3 200 m), 256.29 ± 13.03 (elevation 3 600m ) (P <0.01), and the number of deformity erythrocytes increased significantly. The long-standing altitude plateau group was 368.57 ± 11.26, which was significantly higher than that of the acute plateau group (P <0.01), and the number of deformity erythrocytes was significantly lower than that of the acute plateau group. The number of peripheral blood erythrocytes in the normal form in the Tibetan and Han nationality in the Longhu plateau group was similar (P> 0.05). Conclusion The abnormal changes of peripheral blood red blood cells may be one of the mechanisms of altitude sickness after the normal healthy adults rush into the plateau.