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从病虾中制备病毒粗提液感染日本对虾健康成虾．实验在２０～２４℃水族缸中进行，感染途径分别为头胸甲内近肝胰脏处注射病毒粗提液、投注射病毒粗提液的新鲜牡蛎及直接投病虾肝胰脏．感染７ｄ后死亡率分别为５３．３％、５０％及５５％．利用电镜可在感染致死的对虾肝胰脏中观察到与作为感染源的病虾中形态、大小相同的病毒颗粒．结果表明该病毒可通过饵料由消化道传播，这种病毒感染方法可作为该种病毒人工传代培养的有效方法．
Preparation of virulent crude extract from diseased shrimp infected Japanese shrimp healthy into shrimp. The experiment was carried out in an aquarium with a temperature of 20-24 ° C. The routes of infection were injection of the crude extract of liver and pancreas, fresh oysters of the crude injection of the virus and the hepatopancreas of the shrimps directly into the pancreas. Mortality after 7 days of infection was 53.3%, 50% and 55% respectively. Electron microscopy can be used to identify virus particles with the same morphology and size as those of infected shrimp in hepatopancreas of lethal infected shrimp. The results show that the virus can be transmitted through the digestive tract of food, this virus infection method can be used as artificial transmission of the virus is an effective method.