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目的研究丹酚酸B脂质体的制备方法及其体外释放情况。方法采用逆向蒸发法制备丹酚酸B脂质体,超滤离心法测其包封率,以包封率和粒径为指标,采用正交试验设计法优化处方,并对其表面特征、包封率、粒径、体外释放情况进行考察。结果制备的丹酚酸B脂质的体平均粒径为109.8 nm,药物的平均包封率为71.0%,体外12 h累积释放率为43.7%。脂质体外观圆整而均匀,分散性好。结论制备的丹酚酸B脂质体包封率较高,粒径小,有良好的缓释作用,为后期研究其长循环心靶向脂质体奠定了基础。
Objective To study the preparation of salvianolic acid B liposome and its release in vitro. Methods The liposomal salvianolic acid B was prepared by reverse evaporation method. The entrapment efficiency was determined by ultrafiltration centrifugation. The entrapment efficiency and particle size were used as indexes. The orthogonal design was used to optimize the prescription. The surface features, Gavage rate, particle size, in vitro release were investigated. Results The volume average particle diameter of salvianolic acid B lipids was 109.8 nm. The average entrapment efficiency of drugs was 71.0%. The cumulative release rate of 12 liposomes in vitro was 43.7%. Liposome appearance round and uniform, good dispersion. Conclusion The preparation of salvianolic acid B liposome encapsulation efficiency is high, small particle size, has a good sustained-release effect, for the late study of its long-term cardiac targeting liposomes laid the foundation.