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目的:研究荔枝核提取物体内抗鸭乙型肝炎病毒(DHBV)的作用。方法:应用纯化水或95%乙醇提取荔枝核。将感染DHBV的麻鸭随机分6组,分别给予高剂量水提物(1.4 g.kg-1.d-1)、低剂量水提物(0.35 g.kg-1.d-1)、高剂量醇提物(0.7 g.kg-1.d-1)、低剂量醇提物(0.18 g.kg-1.d-1),以阿昔洛韦(ACV)和纯化水作对照,连续给药10 d,采用荧光定量PCR方法检测给药d0(T0),d 5(T5),d 10(T10)、停药后d4(P4)的DHBVDNA水平。结果:ACV组和高剂量水提物组给药后的DHBVDNA降幅大于纯化水对照组(P值均<0.01)。高剂量水提物组在T10和P4的DHBV DNA低于T0(P<0.01和P<0.05)。结论:荔枝核水提物对DHBV复制有抑制作用。
Objective: To study the anti-duck hepatitis B virus (DHBV) effect in Litchi seed extract. Methods: Litchi seeds were extracted with purified water or 95% ethanol. The ducks infected with DHBV were randomly divided into 6 groups and given high-dose water extract (1.4 g.kg-1.d-1), low-dose water extract (0.35 g.kg-1.d-1), and high A dose of ethanol extract (0.7 g.kg-1.d-1), low-dose alcohol extract (0.18 g.kg-1.d-1), with acyclovir (ACV) and purified water as control, continuous After 10 days of administration, DHBV DNA levels were measured by fluorescent quantitative PCR method at doses of d0 (T0), d 5 (T5), d 10 (T10) and d4 (P4) after drug withdrawal. RESULTS: The decrease in DHBV DNA in the ACV group and the high-dose aqueous extract group was greater than in the purified water control group (P <0.01). In the high-dose aqueous extract group, the DHBV DNA in T10 and P4 was lower than T0 (P<0.01 and P<0.05). CONCLUSION: Litchi seed aqueous extract inhibits DHBV replication.