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目的研究膀胱癌组织中尿激酶型纤维蛋白溶解酶原激活剂(uPA)和基质金属蛋白酶9(MMP-9)表达及与肿瘤微血管生成之间的关系。方法运用免疫组化S-P法检测66例膀胱癌组织和19例膀胱良性病变组织(对照组)中uPA和MMP-9的表达,同时分析不同uPA和MMP-9表达状态下,膀胱癌组织微血管密度(MVD)的变化。结果膀胱癌组织中uPA和MMP-9的表达率分别为72.7%(48/66)和75.8%(50/66),高于对照组中uPA和MMP-9的表达率(分别为26.3%,5/19;和4.21%,8/19),差异有统计学意义(P值分别为<0.001和0.005);浸润性癌中uPA和MMP-9的表达均强于浅表性癌,差异有统计学意义(P值分别为0.046和0.004);低中分化癌中uPA的表达强于高分化癌,差异有统计学意义(P=0.003),MMP-9的表达与肿瘤病理分级无关;uPA表达与MMP-9表达呈显著正相关关系(rs=0.269,P=0.029)。将膀胱癌分为3组,A组为uPA/MMP-9两者均为弱表达(n=14),B组为uPA/MMP-9两者之一为弱表达(n=19),C组为uPA/MMP-9两者均为弱表达(n=33)。3组MVD计数分别为89.9±23.8,71.8±22.0和62.6±31.9,A组MVD计数高于C组,差异有统计学意义(P=0.003)。结论uPA和MMP-9的表达与膀胱癌的浸润和肿瘤微血管生成密切相关,VPA和MMP-9的表达具有协同性,二者的联合检测可作为判断膀胱癌预后的分子标记。
Objective To investigate the relationship between urokinase-type plasminogen activator (uPA), matrix metalloproteinase 9 (MMP-9) and tumor angiogenesis in human bladder cancer. Methods The expressions of uPA and MMP-9 in 66 cases of bladder cancer and 19 cases of benign bladder lesions (control group) were detected by immunohistochemical SP method. The expressions of uPA and MMP-9 in bladder cancer tissue were detected by immunohistochemical SP method. (MVD) changes. Results The expression rates of uPA and MMP-9 in bladder cancer tissues were 72.7% (48/66) and 75.8% (50/66), respectively, which were higher than those in control group (26.3% 5/19; and 4.21%, 8/19 respectively), the differences were statistically significant (P <0.001 and 0.005, respectively). The expression of uPA and MMP-9 in invasive carcinoma was stronger than that in superficial carcinoma (P = 0.046 and 0.004 respectively). The expression of uPA in poorly differentiated cancer was stronger than that in well-differentiated cancer (P = 0.003), and the expression of MMP-9 was not related to tumor grade. UPA There was a significant positive correlation between the expression and the expression of MMP-9 (rs = 0.269, P = 0.029). Urinary bladder cancer was divided into three groups, group A was weakly expressed uPA / MMP-9 (n = 14), group B was weakly expressed uPA / MMP-9 Group uPA / MMP-9 was weakly expressed (n = 33). The MVD counts of the three groups were 89.9 ± 23.8, 71.8 ± 22.0 and 62.6 ± 31.9, respectively. The MVD counts in group A were higher than those in group C, with a significant difference (P = 0.003). Conclusions The expressions of uPA and MMP-9 are closely related to the invasion and tumor angiogenesis in bladder cancer. The expression of VPA and MMP-9 is synergistic. The combined detection of uPA and MMP-9 can be used as a molecular marker for prognosis of bladder cancer.