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为了探索Ｂ．ｔ．ＭＰ—３４２菌株的发酵工艺技术以便进行大规模批量生产．于１９９５～１９９７年间在湖北省农科院 Ｂ． ｔ．研究开发中心对 ＢｔＭＰ— ３４２菌株的发酵工艺技术进行了研究。结果表明：采用 ＭＰ— ３４２菌株和ＹＭ—２Ｓ配方发酵生产液剂、原粉和高含量粉剂具有显著经济效益．ＭＰ－３４２菌株的发酵效价通常可达４ ０００ＩＵ／μＬ以上。采用离心－喷雾干燥工艺制备的ＭＰ３４２原粉．其粉平均效价和晶体含量分别为５５７４４ ＩＵ／μＬ和 １０． ４０％。原粉的物料收率平均为 ３． ５％．效价和晶体平均总收率分别为 ４２． ３０％和 ８３． ３０％。喷雾干燥过程的效价和晶体损失率很小．分别为９．２％和６．１％。ＭＰ－３４２原料贮存过程中稳定性较好。采用发酵液未经浓缩直接喷雾干燥．可获得 ６６ ８００ＩＵ／ｍｇ的原粉．晶体含量为 ７． ６％．原粉收率达 ６．５％，含水量为 ９． ４％，其效价收率和晶体收率分别为９５．７％和９９．８％。发酵液加６．８％ＣaＣＯ_（３）喷雾干燥、菌粉含水量４．５%.菌粉效价为３６ １５３ＩU／ｍｇ，效价收率为 １００．６％、晶体含量３．９％。晶体收率为 １０１．７％。两种工艺的效价和晶体收率均达９５％以上．较离心工
To explore t. MP-342 strain fermentation technology for large-scale mass production. From 1995 to 1997 in Hubei Academy of Agricultural Sciences. t. Research and Development Center of BtMP-342 strains of fermentation technology was studied. The results showed that the MP-342 strain and the YM-2S formula fermenting liquor, original powder and high content of powder have significant economic benefits. Fermentation titer of MP-342 strain is usually more than 4000IU / μL. MP342 raw powder prepared by centrifugal-spray drying process. The average powder potency and crystal content were 55,744 IU / μL and 10, respectively. 40%. Raw material yield average 3. 5%. The average total potency and crystal yield were 42%. 30% and 83. 30%. Spray drying process, the titer and the rate of crystal loss is small. 9.2% and 6.1% respectively. MP-342 raw material storage stability is better. The fermentation broth without spray directly spray drying. Available 66 800IU / mg of the original powder. Crystal content of 7. 6%. The original powder yield of 6.5%, water content of 9. 4%. The potency yield and crystal yield were 95.7% and 99.8% respectively. The fermentation broth was spray-dried with 6.8% CaCO 3, the water content of the powder was 4.5% .The potency of the powder was 36 153IU / mg, the titer was 100.6%, and the crystal content was 3.9%. The crystal yield was 101.7%. The titer and crystal yield of the two processes are both above 95%. Centrifugal workers