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目的了解广州市男男性行为人群(MSM)中,人类免疫缺陷病毒Ⅰ型(HIV-1)亚型流行状况和基因变异特征。方法从2006-2007年广州市发现的、全部19份经男男性传播感染的HIV-1阳性血清样本,应用套式聚合酶链式反应(Nested-PCR)分别扩增gag和env区基因,并测定、分析序列。结果被成功扩增的有17份样本,获得13份env基因序列和17份gag基因序列。经系统进化树分析,11份(64.71%)为CRF01_AE,2份(11.76%)为CRF07_BC,4份(23.53%)为B。各亚型组内基因离散率:在env区,CRF01_AE为(8.27±0.93),CRF07_BC为(11.04±1.72),B为(16.65±1.78);在gag区,CRF01_AE为(5.36±0.68),CRF07_BC为(4.29±1.14),B为(11.75±1.35)。V3环顶端四肽分析显示:8份CRF01_AE和2份CRF07_BC V3环顶端四肽均为GPGQ;3份B亚型中2份为GPGR,1份为GWGR。根据V3环关键氨基酸推测辅助受体使用情况,结果显示:所有样本均被预测使用CCR5辅助受体。结论广州市MSM人群HIV-1感染者中,至少存在CRF01_AE、CRF07_BC 2种重组亚型和B亚型的流行,且大部分流行株可能为使用CCR5辅助受体的巨嗜细胞嗜性/NSI毒株。
Objective To investigate the prevalence and genetic variation of human immunodeficiency virus type 1 (HIV-1) subtype in men who have sex with men (MSM) in Guangzhou. Methods From 19 samples collected from Guangzhou in 2006-2007, all 19 HIV-1 positive serum samples were detected by MSM. The genes of gag and env were amplified by Nested-PCR Determination, analysis of the sequence. Results Seventeen samples were successfully amplified and 13 env genes and 17 gag genes were obtained. Phylogenetic tree analysis showed that 11 (64.71%) were CRF01_AE, 2 (11.76%) were CRF07_BC and 4 (23.53%) were B. In the env region, CRF01_AE was (8.27 ± 0.93), CRF07_BC was (11.04 ± 1.72), B was (16.65 ± 1.78), while in gag region CRF01_AE was (5.36 ± 0.68), CRF07_BC (4.29 ± 1.14) and B (11.75 ± 1.35). Analysis of the top of the V3 loop tetrapeptide revealed that the GPRs of 8 CRF01_AE and 2 copies of the CRF07_BC V3 loop top peptide were both GPGQ; 2 of the 3 B subtypes were GPGR and 1 was GWGR. Based on the key amino acids in the V3 loop, we speculated on the use of co-receptors and the results showed that all samples were predicted to use the CCR5 co-receptor. Conclusions There are at least two CRF01_AE and two CRF07_BC subtypes and two subtypes of B in HIV-1 infected MSM population in Guangzhou, and most of the epidemic strains may be macrophage / NSI poison using CCR5 co-receptor Strain.