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目的观察十溴联苯醚(BDE209)低水平母源性暴露对子一代小鼠学习记忆能力及甲状腺激素分泌的影响。方法无特定病原体级4周龄雌性昆明小鼠自确定交配成功后,随机分为对照组和低、中、高染毒组,每组16只;对照组母鼠灌胃0.01 L/kg体质量花生油,染毒组母鼠根据50、100和300μg/kg体质量灌胃染毒BDE209,1次/d,连续染毒直至子鼠出生21 d,建立妊娠期至哺乳期的暴露模型。染毒结束后,随机选取每组子鼠10只,雌雄各半,观察其体质量及发育状况;采用水迷宫实验检测子鼠的学习记忆能力;采用化学发光免疫分析法测定子鼠血清总三碘甲腺原氨酸(TT3)、游离三碘甲腺原氨酸(FT3)、总四碘甲腺原氨酸(TT4)和游离四碘甲腺原氨酸(FT4)水平;采用比色法测定子鼠海马组织中谷胱甘肽转移酶(GST)和超氧化物歧化酶(SOD)活力、丙二醛(MDA)水平。结果中染毒组子鼠逃避潜伏期长于对照组(P<0.05);高染毒组子鼠逃避潜伏期分别长于对照组和低、中染毒组(P<0.05);高染毒组子鼠平台象限活动时间和穿越平台次数分别少于对照组和低、中染毒组(P<0.05)。随着染毒剂量的增加,子鼠血清中TT3、FT3、TT4和FT4水平逐渐增高(P<0.05),海马组织中GST和SOD活力逐渐降低(P<0.05),MDA水平逐渐增高(P<0.05)。结论低水平母源性BDE209可导致子鼠学习记忆能力下降,并可导致子鼠血清甲状腺激素水平呈剂量依赖性升高,海马组织出现剂量依赖性的氧化应激损伤。
Objective To observe the effects of low level maternal exposure to decabromodiphenyl ether (BDE209) on learning and memory abilities and thyroid hormone secretion in the first generation mice. Methods Four-week-old female Kunming mice without specific pathogen were randomly divided into control group and low, middle and high exposure groups, 16 mice in each group. The control group rats were given 0.01 L / kg body weight Peanut oil and exposure groups were exposed to BDE209 at doses of 50, 100 and 300μg / kg body weight once a day for consecutive 21 days until the birth of the offspring. The exposure model was established during pregnancy and lactation. After the end of the exposure, randomly selected 10 mice per group, male and female, observe their body weight and development status; using water maze test to detect the learning and memory ability of the offspring; using chemiluminescence immunoassay to determine the total serum of the three rats (TT3), free triiodothyronine (FT3), total tetraiodothyronine (TT4) and free tetraiodothyronine (FT4) were detected by colorimetric method. Method to determine the glutathione transferase (GST) and superoxide dismutase (SOD) activity and malondialdehyde (MDA) levels in hippocampus of rats. The results showed that the escape latency of the rats in the treated group was longer than that in the control group (P <0.05). The escape latency of the treated rats in the highly exposed group was longer than that in the control group and in the low and middle exposure groups (P <0.05) Quadrant activity time and the number of crossing the platform were less than the control group and the low and medium exposure groups (P <0.05). The levels of TT3, FT3, TT4 and FT4 in the serum of the offspring rats increased gradually (P <0.05) with the increase of exposure dose. The activities of GST and SOD in hippocampus decreased gradually (P < 0.05). CONCLUSION: Low-level maternal BDE209 can lead to decreased ability of learning and memory in the offspring, and may lead to a dose-dependent increase of serum thyroid hormone level in the offspring rats and a dose-dependent oxidative stress injury in the hippocampus.