论文部分内容阅读
国内外现行乙型肝炎病毒感染的诊断方法,其检测敏感性(大于10~5毒粒/ml)尚未达到最低血清感染水平(10~2毒粒/ml)。本文应用高灵敏度与特异性的DNA多聚酶链反应(PCR),直接检测肝炎患者血清中的HBVDNA,以探讨PCR技术在乙型肝炎病毒感染诊断中的应用及HBVDNA与乙型肝炎血清标志物间的关系。材料与方法检测对象:本组220例,按1990年上海全国病毒性肝炎会议制订的病毒性肝炎防治方案进行诊断,急性肝炎51例,慢性迁延性
At present, the detection sensitivity (greater than 10 ~ 5 virulence / ml) of the current diagnosis of hepatitis B virus infection in China and other countries has not yet reached the lowest serum level (10 ~ 2 virulence / ml). In this paper, high sensitivity and specificity of DNA polymerase chain reaction (PCR), direct detection of serum HBV DNA in patients with hepatitis to explore the use of PCR technology in the diagnosis of hepatitis B virus infection and HBVDNA and hepatitis B serum markers relationship. Materials and Methods Detection of objects: The group of 220 cases, according to the 1990 Shanghai National Viral Hepatitis Conference to develop a program of prevention and treatment of viral hepatitis diagnosis, acute hepatitis in 51 cases, chronic persistent