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【目的】对水稻F8重组自交系群体穗长QTL进行检测,并比较分析相同亲本衍生的不同群体的遗传图谱、QTL位置、QTL效应的异同,鉴定稳定表达的穗长QTL,以期增加对穗长遗传行为的了解,且有助于通过分子聚合育种手段改良穗长性状。【方法】以籼稻品种泸恢99和粳稻品种日本晴(基因组测序)为亲本构建的F8重组自交系群体中的188个家系为研究材料,利用包含207个标记的遗传连锁图谱,采用基于混合线性模型的QTL定位软件QTLNetwork 2.0,对水稻穗长QTL进行定位和效应分析,并比较分析F8、F2群体的QTL定位和遗传图谱异同。【结果】在F8群体中检测到7个与穗长性状相关的QTL,分别位于第2、3、6、7、8、10染色体上,QTL对表型变异的贡献率为3.38%—14.8%,总贡献率为52.5%。F8、F2群体在5条相同染色体上都定位到了穗长QTL,这些QTL所在标记区间物理位置大部分是重叠和包含关系。F8、F2图谱在定位标记数、标记的位置顺序、遗传距离、平均图距等方面发生了变化。【结论】在F8、F2群体检测到一个稳定遗传的主效应QTL位点,位于第6染色体,并发现了4个尚未报道的穗长QTL。
【Objective】 The QTLs for spike length of F8 recombinant inbred lines of rice were detected, and the similarities and differences of genetic maps, QTLs and QTLs of different populations derived from the same parents were compared and analyzed. Long genetic behavior, and help to improve the spike length trait by means of molecular polymerization breeding. 【Method】 A total of 188 families of F8 recombinant inbred lines (Indica rice variety Luhui 99 and Japonica rice variety Nipponbare (Genome Sequencing)) were used as research materials. Genetic linkage map including 207 markers The QTL mapping software QTLNetwork 2.0 was used to locate and analyze the QTLs for spike length in rice. The QTL mapping and genetic map of F8 and F2 were compared and analyzed. 【Result】 Seven QTLs related to panicle length traits were detected in F8 population, which were located on chromosomes 2, 3, 6, 7, 8 and 10 respectively. The contribution rate of QTL to phenotypic variation was 3.38% -14.8% , The total contribution rate of 52.5%. F8 and F2 populations mapped to the ear length QTL on the same 5 chromosomes. Most of the physical locations of the marker loci where these QTLs are located overlap and contain relationships. F8, F2 map in the number of positioning markers, marking the order of the position, genetic distance, the average distance from the map has changed. 【Conclusion】 A stable major QTL locus was detected in F8 and F2 populations on chromosome 6 and four QTLs for panicle length were found.