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目的:应用HBV转基因小鼠动物模型,研究B7-H1对HBsAg免疫效果的影响。方法:用重组人B7-H1与血源性HBsAg联合免疫HBV转基因小鼠,采用ELISA方法观察对转基因小鼠所诱生的HBsAg特异性Th1类细胞因子的影响,ELISPOT方法检测不同免疫方案对小鼠HBsAg特异性分泌IFNγ-T细胞数量的影响,同时检测对小鼠淋巴细胞增殖及血清HBsAb水平的影响。结果:HBsAg组及HBsAg+B7-H1组免疫后脾细胞产生的Th1类细胞因子(IFNγ-、IL-2)、HB-sAg特异性分泌IFNγ-T细胞、T细胞增殖及血清HBsAb水平较对照组显著增加(P<0.05),但HBsAg组及HBsAg+B7-H1组之间无显著性差异。结论:HBsAg可以诱导乙肝转基因小鼠产生高水平Th1类细胞因子,并诱导小鼠产生特异性的体液免疫,打破免疫耐受。B7-H1对HBsAg在HBV转基因小鼠中的免疫效果无影响。
OBJECTIVE: To study the effect of B7-H1 on HBsAg immunization in animal model of HBV transgenic mice. Methods: HBV transgenic mice were immunized with recombinant human B7-H1 and blood-borne HBsAg. The effect of HBsAg-specific Th1-type cytokines induced by transgenic mice was observed by ELISA. ELISPOT was used to detect the effect of different immunization programs on small HBsAg-specific secretion of IFNγ-T cells in mice, meanwhile, the effect on lymphocyte proliferation and serum HBsAb level in mice was also tested. Results: Th1 cytokines (IFNγ-, IL-2) and HBsAg-secreting IFNγ-T cells secreted by spleen cells in HBsAg group and HBsAg + B7-H1 group were higher than those in control group Group (P <0.05), but there was no significant difference between HBsAg group and HBsAg + B7-H1 group. Conclusion: HBsAg can induce high level of Th1 cytokines in hepatitis B transgenic mice and induce specific humoral immunity in mice to break immune tolerance. B7-H1 had no effect on the immunogenicity of HBsAg in HBV transgenic mice.