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背景:有关决明子降脂的有效化学成分及作用途径作者进行资料查询时尚未找到明确报道。目的:观察决明子蒽醌类化合物对实验性高脂血症大鼠血脂水平及细胞内源性总胆固醇合成的影响,推断决明子降脂的有效成分及作用途径。设计、时间及地点:随机对照动物实验,于2003-09/2004-05在华南师范大学生命科学学院完成动物饲养及实验,在生物技术研究所完成尾静脉取血、血脂测定、细胞培养及存活率测定。材料:选用SD雄性成年大鼠45只,均灌胃脂肪乳剂建立高脂血症动物模型。决明子购于广州药房,由华南植物研究所鉴定;蒽醌由华南师范大学生物技术研究所制备。方法:将45只SD雄性大鼠按随机数字表法分为3组(n=15):即对照组、决明子蒽醌80mg/kg组和决明子蒽醌20mg/kg组。造模2d后各组动物每天上午继续灌胃脂肪乳剂1次;下午按实验分组灌胃相应剂量的药物,对照组灌胃相同剂量的生理盐水,1次/d,连续20d。主要观察指标:应用两性霉素B细胞模型法检测决明子蒽醌在中国仓鼠卵细胞内源性胆固醇合成中的作用。应用酶终点法测定各组大鼠血清总胆固醇、三酰甘油、高密度脂蛋白胆固醇及低密度脂蛋白胆固醇水平;采用四甲基偶氮唑蓝法以分光光度测定中国仓鼠卵细胞的存活率(A570)。结果:①决明子蒽醌能明显降低高脂血症大鼠血清总胆固醇、三酰甘油及低密度脂蛋白胆固醇水平,提高高密度脂蛋白胆固醇水平,呈明显的量-效关系。②决明子蒽醌能提高中国仓鼠卵细胞的存活率。结论:决明子蒽醌具有降血脂作用,是决明子降脂的主要有效部位;抑制细胞内胆固醇的合成可能是决明子蒽醌降脂的作用途径之一。
BACKGROUND: The effective chemical composition and action of Cassia lucidum for reducing lipids have not been clearly reported by the authors when they conducted data inquiries. OBJECTIVE: To observe the effect of cassia cassia compound on serum lipid levels and the synthesis of endogenous total cholesterol in experimental hyperlipidemia rats, and to infer the effective components of cassia seed lipid lowering and its action pathway. DESIGN, TIME AND SETTING: A randomized controlled animal experiment was conducted at the Life Science College of South China Normal University from September 2003 to May 2004. Blood sampling, lipid measurement, cell culture, and survival were performed at the Institute of Biotechnology. Rate determination. MATERIALS: Forty-five adult male Sprague-Dawley rats were used, and fat emulsions were intragastrically administered to establish an animal model of hyperlipidemia. Cassia was purchased from a pharmacy in Guangzhou and was identified by the South China Institute of Botany. The triterpenes was prepared by the Institute of Biotechnology, South China Normal University. Methods: Forty-five SD male rats were divided into three groups according to the random number table (n=15): control group, cassia seed meal 80 mg/kg group and cassia seed meal 20 mg/kg group. After 2 days of modeling, animals in each group continued to infuse fat emulsion once a day; in the afternoon, corresponding doses of drugs were given by gavage; the control group was given the same dose of normal saline once a day for 20 days. MAIN OUTCOME MEASURES: The role of cassia seed meal in the synthesis of endogenous cholesterol in Chinese hamster ovary cells was detected using the amphotericin B cell model. The levels of total cholesterol, triacylglycerol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were measured by enzyme end point method; the survival rate of Chinese hamster ovary cells was measured by spectrophotometry using tetramethylazol blue ( A570). RESULTS: 1 Cassia jasminoides could significantly reduce serum total cholesterol, triacylglycerol and low-density lipoprotein cholesterol levels in hyperlipidemic rats, and increase high-density lipoprotein cholesterol levels, showing a significant dose-effect relationship. 2 Cassia wolfberry can increase the survival rate of Chinese hamster ovary cells. Conclusion: Cassia jasminoides has a hypolipidemic effect, which is the main effective site of lipid-lowering of cassia seed. Inhibition of intracellular cholesterol synthesis may be one of the ways of reducing lipids of cassia seedlings.