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探讨紫苏甲醇提取物(PLME)的自由基清除能力和对过氧化氢诱发人类角质形成细胞(HaCaT)细胞氧化损伤的保护作用。细胞经氧化损伤造模后,换用含不同浓度(50~200μg/mL)的PLME继续培养24h,MTT法检测细胞生存率,硫代巴比妥酸比色法测定MDA含量。DPPH和羟基自由基清除实验测定PLME的体外自由基清除能力。PLME干预氧化损伤细胞后,细胞生存率上升,且MDA生成量减少,与未干预组相比较差异有统计学意义(P<0.01),并显示出剂量效应关系。此外,PLME对DPPH和羟基自由基有强清除能力,其对DPPH自由基半清除剂量IC50=9.07μg/mL,对羟基为IC50=49.28μg/mL。结果提示,PLME具有较强的自由基清除能力,同时还能有效地减少氧化损伤下HaCaT细胞内MDA生成,提高细胞生存率,对细胞应激损伤有保护作用。
To investigate the free radical scavenging activity of perilla methanol extract (PLME) and the protective effect of hydrogen peroxide on the oxidative damage of human keratinocytes (HaCaT). The cells were treated with PLME at different concentrations (50 ~ 200μg / mL) for 24 hours. Cell viability was measured by MTT assay and MDA content by thiobarbituric acid colorimetric assay. DPPH and hydroxyl free radical scavenging assay PLME in vitro free radical scavenging capacity. After PLME intervention, the cell survival rate increased and MDA production decreased, which was significantly different from that of the non-intervention group (P <0.01) and showed a dose-response relationship. In addition, PLME has a strong ability to scavenge DPPH and hydroxyl radicals. The IC50 of DPPH for free radical scavenging DPPH is 9.07μg / mL and IC50 = 49.28μg / mL for hydroxyl group. The results suggest that PLME has a strong ability of free radical scavenging, meanwhile it can effectively reduce the MDA production in HaCaT cells under oxidative damage, improve the cell survival rate and protect cell stress injury.