论文部分内容阅读
目的揭示重庆地区肝癌患者ＨＢＶ和／或ＨＣＶ的感染与癌组织中癌基因和抑癌基因表达之间的联系。方法应用免疫组织化学方法检测 ＨＢＶ和 ＨＣＶ抗原以诊断 ＨＢＶ和 ＨＣＶ感染，癌基因 ｒａｓ、ｃ－ｍｙｃ、ｃ－ｅｒｂＢ－２和抑癌基因 ｐ５３蛋白的表达也用免疫组化方法检测。结果 ４６例肝癌患者中，ＨＢＶ感染３４例，ＨＣＶ感染２０例；其癌组织中ｒａｓ ｐ２１、ｃ－ｍｙｃ、ｃ－ｅｒｂＢ－２和ｐ５３蛋白表达阳性率分别为 ５８．７％、６７．４％、４７．８％和 ３０．４％，ＨＣＶ双重感染１４例，单独 ＨＢＶ感染 ２０例，单独 ＨＣＶ感染 ６例，无 ＨＢＶ、ＨＣＶ感染 ６例；四组不同 ＨＢＶ、ＨＣＶ感染状况肝癌组织中ｒａｓｐ２１、ｃ－ｍｙｃ、ｃ－ｅｒｂＢ－２和 ｐ５３蛋白表达阳性率无显著差异（ Ｐ＞０．０５）。结论 ＨＢＶ、ＨＣＶ感染可能与原发性肝癌发生密切相关，ＨＢＶ、ＨＣＶ的不同感染状况与癌基因ｒａｓ、ｃ一ｍｙｃ、ｃ－ｅｒｂＢ－２和抑癌基因ｐ５３蛋白表达之间的联系并不明显。
Objective To reveal the association between HBV and / or HCV infection in hepatocellular carcinoma patients and the expression of oncogenes and tumor suppressor genes in cancer tissues in Chongqing area. Methods The HBV and HCV antigens were detected by immunohistochemistry in order to diagnose HBV and HCV infection. The expressions of oncogenes ras, c-myc, c-erbB-2 and tumor suppressor gene p53 protein were also detected by immunohistochemistry. Results The positive rate of ras p21, c-myc, c-erbB-2 and p53 protein expression in cancer tissues was 58.7% and 67.4% respectively in 34 cases of hepatocellular carcinoma and in 20 cases of HCV infection. , 47.8% and 30.4% respectively. There were 14 cases of HCV double infection, 20 cases of HBV infection alone, 6 cases of HCV infection alone, 6 cases without HBV and HCV infection. The positive rates of c-myc, c-erbB-2 and p53 were not significantly different (P> 0.05). Conclusions HBV and HCV infection may be closely related to the occurrence of primary hepatocellular carcinoma. The relationship between different infection states of HBV and HCV and the expression of oncogenes ras, c-myc, c-erbB-2 and tumor suppressor gene p53 protein is not obvious .