论文部分内容阅读
将结核分枝杆菌(结核杆菌)H37连续传代可得到减毒株H37Ra与保持完整毒力的有毒株H37Rv。作者对两者进行基因组对比研究,并确定变异基因位点,旨在说明H57R毒力减弱的分子机制。用稀有切点的限制性内切酶DraⅠ对H37Rv和H37R进行酶切后,经脉冲电场凝
Successive passage of M. tuberculosis (Mycobacterium tuberculosis) H37 results in the attenuated strain H37Ra and the virulent strain H37Rv that remains intact. The authors conducted a genome-wide comparative study of the two and determined the site of the mutated gene to elucidate the molecular mechanism underlying the attenuated virulence of H57R. H37Rv and H37R were digested with restriction endonuclease Dra I