论文部分内容阅读
Aim:To classify the genes responsible for apoptosis in QGY-7703 cells in-duced by homoharringtonine (HHT).Methods:Apoptosis in QGY-7703 cellsinduced by HHT was demonstrated by DNA fragmentation and morphologicalobservation,cDNA microarray technology was used to detect gene transcription,and the result of microarrays for genes was confirmed by RT-PCR.Results:Seventy-eight individual mRNA were identified and their transcription levelschanged significantly.Those genes,of which 68% were upregulated and 32%were downregulated,were partially related to apoptosis.They were mostlyoncogenes,tumor suppressors,enzymes,and kinases.Conclusion:HHT is apotential drug in the treatment of liver cancer.TGF-β,TNF,FAS,p38MAPK,and p53 apoptosis signaling pathways were activated during apoptosis in QGY-7703 cells.Such inducible genes may play important roles in apoptosis anddeserve to be further studied.
Aim: To classify the genes responsible for apoptosis in QGY-7703 cells in-duced by homoharringtonine (HHT). Methods: Apoptosis in QGY-7703 cells induced by HHT was demonstrated by DNA fragmentation and morphologicalobservation, cDNA microarray technology was used to detect gene transcription , and the result of microarrays for genes was identified by RT-PCR. Results: Seventy-eight individual mRNAs were identified and their transcription levels changed significantly. Thy genes, of which 68% were upregulated and 32% were downregulated, were partially related to apoptosis They were mostly oncogenes, tumor suppressors, enzymes, and kinases. Conflux: HHT is apotential drug in the treatment of liver cancer. TGF-β, TNF, FAS, p38MAPK, and p53 apoptosis signaling pathways were activated during apoptosis in QGY-7703 cells .Such inducible genes may play important roles in apoptosis anddeserve to be further studied.