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目的:采用骨髓基质细胞(BMSCs)体内修复髁突软骨全层缺失。方法:15只山羊,9只作为实验组,将BMSCs和少量软骨细胞(7:3比例混合)按5×107/mL与生物可降解材料复合后,植入山羊髁突软骨全层缺失处;对照组6只山羊,髁突软骨全层缺失区植入支架材料,分别于术后4、8、12周每个时间段取材3只实验动物,2只对照组动物;2组分别用HE染色、Ⅱ型胶原分泌的免疫组化法进行评价。结果:实验组术后4周,山羊髁突软骨缺失区能形成成熟的软骨组织,12周时软骨未退变。对照组不能形成成熟的软骨组织。结论:骨髓基质细胞在自体软骨细胞基质的诱导下,可以修复山羊颞下颌关节髁突软骨面全层缺失。
OBJECTIVE: To use bone marrow stromal cells (BMSCs) to repair condylar cartilage defects in vivo. Methods: Fifteen goats were used as experimental group. BMSCs and a small amount of chondrocytes (7: 3 ratio) were mixed with biodegradable material at 5 × 107 / mL and then implanted into the condylar cartilage in the absence of full thickness. In the control group, 6 goats and scaffolds were implanted into the full-thickness defect area of condylar cartilage. Three experimental animals and two control animals were harvested at each time period of 4, 8 and 12 weeks after operation. Two groups were stained with HE , Type Ⅱ collagen secretion immunohistochemistry for evaluation. Results: At 4 weeks after operation, mature cartilage tissue was formed in the condylar cartilage missing region in the experimental group, and did not degenerate after 12 weeks. Control group can not form mature cartilage tissue. CONCLUSION: Bone marrow stromal cells can repair the condylar chondral surface of goat temporomandibular joint under the induction of autologous chondrocytes.