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本文介绍一种简便、变性现象少的姊妹染色单体分化染色法—BrdU-AG法。将末梢血淋巴细胞加入20%胎牛血清于RPMI-1640培养基中,培养72小时。在培养终止前24小时添加BrdU,其最终浓度为2～6微克/升。碱性Giemsa液是用10%NaOH调pH7.2的磷酸缓冲液至pH11,再加入适量的Giemsa液,配制成1.8%的碱性Giemsa液用来染色,按常规方法制成的染色体标本玻片置于1.8%碱性Giemsa染液中5～10分钟即
This article presents a simple, less degenerate phenomenon of sister chromatid staining - BrudU-AG method. Peripheral blood lymphocytes were added to 20% fetal bovine serum in RPMI-1640 medium and cultured for 72 hours. BrdU was added 24 hours prior to the termination of culture, with a final concentration of 2 to 6 micrograms per liter. Alkaline Giemsa solution is adjusted to pH 11 with 10% NaOH pH7.2 phosphate buffer and then added to the appropriate amount of Giemsa solution to prepare a 1.8% alkaline Giemsa solution for staining. Placed in 1.8% alkaline Giemsa dye solution for 5 to 10 minutes