,Translocation of classical PKC and cortical granule exocytosis of human oocyte in germinal vesicle

• 作 者: Xue-qing WU Xiao ZHANG Xiao-hong LI Hui-hong CHENG Yan-rong KUAI Song WANG Ying-lu GUO
• 机 构: Institute of Urology, Peking University, Beijing 100034, China“,”Reproduction and Genetic Center, Pe
• 出 处: 中国药理学报（英文版）
• 发表日期: 2006年10期
• 关键词: cortical granule human oocyte protein kinase C

Aim: Protein kinase C (PKC) is as a family of serine/threonine kinases that can be activated by Ca2+, phospholipid and diacylglycerol. PKC plays an important role in oocyte maturation and activation. This study was undertaken to investigate classical PKC (cPKC) in human oocyte maturation and activation. Methods: Germinal vesicle (GV) and metaphase Ⅱ (MII) stage oocytes were collected from healthy women. The expression and distribution of cPKC were investigated by immunoflourescence. MII oocytes were treated with PKC activator or inhibitor and imaged using a laser confocal scanning microscope (LCSM). Results: In GV oocytes, PKC α,β1 and γ were localized to the germinal vesicles, with a weak expression in ooplasm. In MII oocytes, PKCα, β1 and γ were distributed evenly in ooplasm. After treatment with PKC activator, phorbol 12-myristate 13-acetate (PMA), cPKC translocated to the periphery of oocyte, and cortical granules (CG) exocytosis was found. When the oocytes were treated with PKC inhibitor, staurosporine, no translocation of cPKC and CG exocytosis were found. Conclusion: PKCα, β1 and γ exist in human oocytes and activation of these subunits could induce CG exocytosis in MII stage.