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研究回心草活性部位分离的单体成分对人脐静脉内皮活细胞数、内皮细胞NO及NO合酶的影响,探讨回心草抗动脉硬化的药理作用机制。采用H2O2建立体外培养的HUVEC操作模型,用噻唑蓝(MTT)法测定5个单体对H2O2损伤的内皮细胞的光密度(OD);用试剂盒检测各剂量组细胞上清液中一氧化氮浓度和细胞内一氧化氮不同种类的合成酶的活性。结果表明H2O21mmol/L为最合适的细胞刺激浓度;回心草活性部位的5个单体成分药液与H2O2损伤的血管内皮细胞共同孵育24h后,与模型组比较细胞抑制率均有显著差异(P<0.05或P<0.01);进行一氧化氮、一氧化氮合成酶含量的测定,与模型组比较亦有显著差异(P<0.05或P<0.01)。所以,回心草活性部位的5个单体成分直接作用于H2O2损伤的内皮细胞,均可以减轻内皮细胞的损伤,增加NOS活性,促进NO的分泌。
To study the effect of monomer components isolated from the active site of R. officinalis on the number of living cells of human umbilical vein endothelial cells, NO and NO synthase in endothelial cells, and to explore the pharmacological mechanism of R. officinalis against arteriosclerosis. HUVEC operation model was established with H2O2 in vitro. Optical density (OD) of 5 monomer-damaged endothelial cells was measured with MTT method; Nitric oxide in cell supernatants was measured in each dose group by kit. Concentration and intracellular nitric oxide activity of different types of synthetases. The results showed that H2O21 mmol/L was the most suitable cell stimulating concentration; the five monomeric components of R. officinalis incubating with H2O2 injured vascular endothelial cells for 24 h were significantly different from the model group in cell inhibition rate. P<0.05 or P<0.01); Nitric oxide and nitric oxide synthase levels were measured and compared with those in the model group (P<0.05 or P<0.01). Therefore, the five monomeric components of the active part of R. officinalis act directly on H2O2-damaged endothelial cells, which can all reduce endothelial cell injury, increase NOS activity, and promote NO secretion.