论文部分内容阅读
目的:探讨苦豆碱对氧糖剥夺再灌注损伤后海马神经元的保护作用。方法:以原代培养的新生大鼠海马神经元为研究对象建立氧糖剥夺再灌注损伤模型,MTT法检测神经细胞的存活率,分光光度法检测乳酸脱氢酶(LDH)的漏出率,激光共聚焦显微镜(CLSM)测定海马神经元细胞内Ca2+浓度和线粒体膜电位水平(MMP),利用hoechst33342检测细胞凋亡率。结果:与氧糖剥夺再灌注损伤组比较,苦豆碱(25,50,100 mg/L)组可显著提高细胞存活率,降低乳酸脱氢酶漏出率,抑制Ca2+浓度升高,苦豆碱(25,50,100 mg/L)组能显著降低线粒体膜电位水平,降低细胞凋亡率。结论:苦豆碱对海马神经元氧糖剥夺再灌注损伤具有明显的保护作用。
Objective: To investigate the protective effects of aloperine on hippocampal neurons after oxygen-glucose deprivation and reperfusion injury. Methods: The primary cultured neonatal rat hippocampal neurons were used to establish the models of oxygen-glucose deprivation-reperfusion injury. The survival rate of neurons was detected by MTT assay. The leakage rate of lactate dehydrogenase (LDH) was detected by spectrophotometry. Confocal microscopy (CLSM) was used to measure intracellular Ca2 + concentration and mitochondrial membrane potential (MMP) in hippocampal neurons, and the apoptosis rate was detected by hoechst33342. Results: Compared with oxygen deprivation and reperfusion injury groups, the treatment with oxymatrine (25, 50, 100 mg / L) significantly increased the cell viability, decreased the leakage rate of lactate dehydrogenase, and inhibited the increase of Ca2 + concentration. , 50,100 mg / L) could significantly reduce the level of mitochondrial membrane potential and reduce the rate of apoptosis. CONCLUSION: Hyperaritine has a significant protective effect on oxygen-glucose deprivation-reperfusion injury in hippocampal neurons.