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本文观察了热应激对大鼠肺组织细胞膜中β-受体Bmax,磷脂酶A_2(PLA_2)活性,磷脂酰胆碱(PC),磷脂酰丝氨酸(PS)含量的影响及膜流动性的改变。测得正常大鼠肺中β-受体的B_(max),PLA_2活性,PC和PS值分别为479±94fmol/mg蛋白,78.5±8.2U,53.5±9.7mg/g湿重和425.1±68.1μg/g湿重;在热应激使肛温达42℃并持续15min时,β-受体B_(max)减少43％;PLA_2活性增加83％,PC和PS分别减少50％和47％;测流动性结果表明,热应激使红细胞和肺组织细胞膜流动性都明显减弱。作者认为这些变化之间的关系是:β-受体B_(max)的减少可能是热应激时激活了肺组织细胞膜中的PLA_2,加速了PC和PS等的分解代谢,使膜液晶态改变的结果。
In this paper, the effects of heat stress on the expression of β-receptor Bmax, PLA 2, phosphatidylcholine (PC) and phosphatidylserine (PS) in the lung tissue of rats and the changes of membrane fluidity . The B max and PLA 2 activities of β-receptors in the lung of normal rats were measured. The values of PC and PS were 479 ± 94 fmol / mg protein, 78.5 ± 8.2 U, 53.5 ± 9.7 mg / g wet weight and 425.1 ± 68.1 μg / g wet weight; the β-receptor B max decreased by 43% when the rectal temperature reached 42 ℃ for 15 min under heat stress; the PLA 2 activity increased by 83% and the PC and PS decreased by 50% and 47%, respectively; Measurement of fluidity results show that heat stress to red blood cells and lung tissue membrane fluidity were significantly weakened. The authors believe that the relationship between these changes is: β-receptor B max (max) reduction may be activated by heat stress in the lung tissue membrane PLA 2, accelerated PC and PS catabolism, the membrane liquid crystal change the result of.