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材料与方法:①体内抗流感病毒实验:BALB/c小鼠,雌性。小鼠腹腔内注射戊巴比妥钠麻醉,于鼻腔滴入1μl pH为7.2且含0.1％牛血清的小鼠敏感流感病毒A/PR/8/34的PBS悬液,于3～7d后感染从鼻腔粘膜扩散至肺部。②建立过敏性肺炎小鼠模型:BALB/c小鼠,雌性,6周龄。腹腔内注射含有卵白蛋白(OVA)8μg和明矾2mg的生理盐水注射液0.5ml,5d后明矾OVA注射液加强注射1次,未致敏动物只使用明矾。致敏7d后,每隔4h让动物吸
Materials and Methods: 1 In vivo anti-influenza virus experiment: BALB/c mice, female. The mice were anesthetized with sodium pentobarbital by intraperitoneal injection and 1 μl of a PBS suspension of mouse sensitive influenza virus A/PR/8/34 with a pH of 7.2 and containing 0.1% bovine serum was instilled into the nasal cavity and infected 3 to 7 days later. Difuse from the nasal mucosa to the lungs. 2 Establish a mouse model of allergic pneumonia: BALB/c mice, female, 6 weeks old. Intraperitoneal injection of 0.5 ml of physiological saline injection containing OVA 8 μg and alum 2 mg was given. The alum was injected with OVA for 1 injection. The unsensitized animals used only alum. After 7 days of sensitization, the animal was sucked every 4 hours