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目的 研究催乳素 (PRL)对人甲状腺细胞表面HLA DR和CD4 0表达的调节作用 ,以探讨PRL影响Graves’病(GD)发生、发展的可能机制。方法 将传代培养的GD和对照组 (取自结节性甲状腺肿和甲状腺腺瘤病变周围组织 )甲状腺细胞分别经羊PRL(oPRL)、IFN γ或IL 4以及oPRL和IFN γ(10U/ml)或IL 4 (5ng/ml)作用 7天。利用免疫荧光染色和流式细胞仪测定其表面HLA DR和CD4 0的表达。结果 oPRL(12 5 - 10 0 0ng/ml)对甲状腺细胞表面HLA DR和CD4 0的表达均没有明显的直接影响。而且它对IFN γ刺激两组甲状腺细胞及IL 4刺激GD甲状腺细胞表达HLA DR的作用亦无明显影响。oPRL能够拮抗IFN γ对两组甲状腺细胞表面CD4 0表达的刺激作用和IL 4的抑制作用。此拮抗作用与PRL的浓度有关。在 2 0 0 (GD :P <0 0 1及P <0 0 5 ;对照组 :P <0 0 5 )和 10 0 0ng/ml(P <0 0 1及P <0 0 5 )时 ,它能使IFN γ刺激的阳性细胞百分率及平均荧光强度 (dMF)均显著减少。在 12 5和 10 0 0ng/ml时能使受到IL 4抑制作用的两组甲状腺细胞中CD4 0 +细胞百分率以及在 10 0 0ng/ml时能使GD甲状腺细胞相应的dMF明显增加 (P <0 0 5 )。对于后者 ,与IL 4单独作用时比 ,oPRL在 12 5和 10 0 0ng/ml时GD甲状腺细胞的dMF明显增加 (P <0 0 5 )。结?
Objective To study the regulatory effect of prolactin (PRL) on the expression of HLA DR and CD4 0 on human thyrocytes, and to explore the possible mechanism of PRL on the occurrence and development of Graves’ disease (GD). Methods Thyroid cells from subcultured GD and control groups (derived from nodular goiter and thyroid adenoma surrounding tissues) were treated with sheep PRL (oPRL), IFNγ or IL 4 and oPRL and IFN γ (10U / ml) Or IL 4 (5 ng / ml) for 7 days. Immunofluorescence staining and flow cytometry were used to detect the expression of HLA DR and CD4 0. Results oPRL (125 - 10 0 ng / ml) had no significant direct effect on the expression of HLA DR and CD4 0 on thyroid cells. Moreover, it also had no significant effect on IFNγ-stimulated thyroid cells and IL-4-stimulated GD thyroid cells to express HLA-DR. oPRL could antagonize the stimulatory effect of IFNγ on the CD4 0 expression on the surface of thyrocytes and IL 4 inhibition. This antagonism is related to the concentration of PRL. At 200 (GD: P <0.01 and P <0.05; control: P <0.05) and 100 ng / ml (P <0.01 and P <0.05) The percentage of positive IFN-γ-stimulated cells and the mean fluorescence intensity (dMF) were all significantly reduced. The percentages of CD4 + cells in two groups of thyrocytes under IL-4 inhibition and the corresponding dMF at 100ng / ml of GD thyroid cells were significantly increased at 125 and 100 ng / ml (P <0 0 5). For the latter, the dMF of GD thyroid cells was significantly increased at oPRL at 125 and 100 ng / ml (P <0.05) compared with IL4 alone. Knot?