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目的 :探讨大剂量60 Coγ射线事故照射和离体照射对人精子与淋巴细胞染色体畸变的影响。方法 :应用人精子穿透金黄地鼠卵获得精子中期分裂相和微量全血培养法制备淋巴细胞中期分裂相 ,分析两例在60 Co辐射事故中全身受到大剂量γ射线照射的急性放射病患者照后 6( 7)年的精子和淋巴细胞染色体畸变 ,以及60 Coγ射线离体照射人精子与淋巴细胞诱发染色体畸变 ,并对全身照射与模拟离体照射进行比较。结果 :两例事故受照患者的畸变精子率显著高于对照组 ,亦显著高于同期淋巴细胞染色体畸变水平 ;两患者精子染色体断裂型畸变是重接型的 2倍 ,而淋巴细胞重接型畸变是断裂型的 2倍 ,两患者的Y 精子与X 精子的比例明显增高 ( 1 60∶1)。在离体照射条件下 ,人精子和淋巴细胞的畸变细胞率基本相同 ,均与照射剂量呈正相关 ;结构畸变类型明显不同 ,精子染色体断裂型畸变是重接型的 8倍 ,淋巴细胞重接型畸变是断裂型的 4倍 ;Y 精子与X 精子的比例为 1∶1。在相同剂量点 ,模拟照射组的人精子染色体畸变率显著高于全身照射组 ,两受照组精子染色体均以断裂型畸变为主 ,但事故照射组精子重接型畸变的比例显著高于离体照射组。结论 :在全身照射情况下 ,电离辐射对生殖细胞影响的重点是精原干细胞 ,而且在精子发生的过程中有
Objective: To investigate the effects of high dose 60 Co γ-ray irradiation and chromosomal aberrations on human sperm and lymphocytes. Methods: The human metaphase spermatozoa were dissected through the golden hamster ovum to obtain the metaphase of lymphocytes and the mid-schizophrenia of the whole spermatozoa. Two cases of acute radiation sickness patients who were exposed to high doses of γ-rays throughout the 60Co radiation accident were analyzed Chromosomal aberrations of sperm and lymphocytes after 6 (7) years and chromosomal aberration induced by 60 Co γ -ray irradiation in human spermatozoa and lymphocytes were compared with that of whole body irradiation and simulated ex vivo irradiation. Results: The aberration rate of sperm in two incident patients was significantly higher than that in control group, and also significantly higher than that of lymphocyte in the same period. The chromosome aberration of sperm in two patients was twice that of reconnection, Distortion is two times the type of fracture, the two patients significantly increased the proportion of Y sperm and X sperm (1 60: 1). Under the condition of in vitro irradiation, the rate of aberrant cells in human sperm and lymphocytes were almost the same, which were positively correlated with the dose of irradiation. The types of structural aberrations were obviously different. The chromosome aberration of sperm chromosome was 8 times that of reconnection, Distortion is 4 times the breaking type; Y sperm and X sperm ratio of 1: 1. At the same dose, the rate of human sperm chromosome aberration in simulated irradiation group was significantly higher than that of the whole body irradiation group. The chromosomes of the two irradiated groups were mainly fracture-type, but the proportion of sperm reconnection-type distortion in the irradiation group was significantly higher than that of the normal group Body irradiation group. Conclusion: In the case of whole body irradiation, the focus of ionizing radiation on germ cells focuses on spermatogonial stem cells, and during spermatogenesis