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目的建立HPLC同时测定青娥丸中12种指标成分的方法,研究不同炮制品配伍的青娥丸中指标性成分含量变化。方法色谱柱:Purospher~ STAR LPRP-C_(18)endcapped(250 mm×4.6 mm,5μm);流动相:0.1%甲酸水-乙腈梯度洗脱;流速:1.0 m L·min~(-1);检测波长:240 nm;进样量:10μL;柱温:30℃。对不同炮制品配伍的青娥丸中指标性成分进行含量测定。结果在上述色谱条件下,青娥丸中12个成分分离度良好,加样回收率范围96.04%~104.07%,RSD均小于2.95%。盐炙品青娥丸中补骨脂素、异补骨脂素、补骨脂定、补骨脂二氢黄酮甲醚、Corlifol A、补骨脂酚、异补骨脂查尔酮7种成分含量明显下降(P<0.05,P<0.01,P<0.001),其他成分无明显变化。结论本研究所建立的方法简单,准确性和稳定性较好,可用于青娥丸的质量控制。盐炙品配伍的青娥丸中补骨脂素、异补骨脂素等成分与生品配伍的青娥丸比较含量明显下降。
OBJECTIVE To establish a method for the simultaneous determination of 12 kinds of indicators in Qing-e-Pill by HPLC and to study the changes of the index components in Qing-e-Pang with compatibility of different processed products. METHODS: Purospher ~ STAR LPRP-C_ (18) endcapped (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase was eluted with 0.1% formic acid in water and acetonitrile. The flow rate was 1.0 m L · min ~ ; Detection wavelength: 240 nm; injection volume: 10 μL; column temperature: 30 ℃. The content of index components in Qing’e pills with different processed products was determined. Results Under the above chromatographic conditions, 12 components of Qing-E were separated well, with the recoveries of 96.04% -104.07% and RSDs less than 2.95%. Salt sunburn products Qing’e pills psoralen, isopsoralen, psoralen, psoralen, Corlifol A, bakuchiol, isopsoralen Chalcone seven kinds of ingredients (P <0.05, P <0.01, P <0.001), while the other components did not change significantly. Conclusion The method established in this study is simple, accurate and stable, and can be used for the quality control of Qing-e-Pill. Salt Sunburn compatibility of Qing e pill in psoralen, isopsoralen and other ingredients and green tea compatibility of the content significantly decreased.