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A new approach used to measure pre-cancer-induced genetic damage in a patient diagnosed as early B-precursor acute lymphoblastic leukemia, by totally separating the malignant B-lymphocytes and the normal T-lymphocytes is described. The lymphocytes separation by a rosetting method and chromosome transloeation analysis using chromosome painting were employed, in the case presented here, the utility of this approach is illustrated using blood lymphocytes from a nuclear dockyard worker who claims that his leukemia was induced by work-related radiation exposures. Blood lymphocytes were obtained after diagnosis of the disease, but prior to therapy, and measurements made of (1) the transloeation frequency in separated normal T-lymphocytes and (2) the trauslocation frequency in phytohemagglutinin (PHA) stimulated lymphocytes,which include a fraction of the malignant B cells. The approach described here makes possible biodosimetry of pre-cancer exposures in these patients and may provide the dosimetric informatio
A new approach used to measure pre-cancer-induced genetic damage in a patient diagnosed as early B-precursor acute lymphoblastic leukemia, by separating the malignant B-lymphocytes and the normal T-lymphocytes is described. The lymphocytes separation by a rosetting method and chromosome transloeation analysis using chromosome painting were employed, in the case presented here, the utility of this approach is illustrated using the blood lymphocytes from a nuclear dockyard worker who claims that his leukemia was induced by work-related radiation exposures. Blood lymphocytes were acquired after diagnosis of the disease, but prior to therapy, and measurements made of (1) the transloeation frequency in separated normal T-lymphocytes and (2) the trauslocation frequency in phytohemagglutinin (PHA) stimulated lymphocytes, which include a fraction of the malignant B cells The approach described here makes possible biodosimetry of pre-cancer exposures in these patients and may provide the do simetric informatio