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目的 研究膀胱癌微转移的情况及临床意义。方法 以靶向细胞角蛋白 2 0 (Cyokeratinck 2 0 )逆转录多聚酶链反应 (RT PCR)检查 91例膀胱癌微转移情况。结果 91例膀胱癌中检出CK 2 0mRNA37例 ,阳性率 41% ,其中证实有转移的 2 0例中 17例阳性 ( 85 % ) ,发现无淋巴结转移的Ⅰ～Ⅱ期患者行TRRBP术后 15个月 2例复发者CK 2 0 mRNA均表达阳性 ;2 5例健康对照组均为阴性。结论CK 2 0RT PCR较常规组织学检查具有更高敏感性 ,提高了微转移的检出率 ,对判断患者预后 ,指导术后辅助治疗具有重要意义。
Objective To study the micro-metastasis of bladder cancer and its clinical significance. Methods Ninety-one micrometastases of bladder transitional cell carcinoma were detected by reverse transcription-polymerase chain reaction (RT-PCR) targeting cytokeratin 20. Results Among 91 cases of bladder cancer, 37 cases were found CK20 mRNA, the positive rate was 41%. Among the 20 cases, 17 cases were positive (85%), of which there was no lymph node metastasis in stage I ~ II patients. TRRBP 15 The CK 2 0 mRNA expression was positive in 2 cases of recurrence in every month. All 25 healthy controls were negative. Conclusion CK20R PCR has higher sensitivity than routine histological examination and improves the detection rate of micrometastasis, which is of great significance in judging the prognosis of patients and guiding postoperative adjuvant therapy.