毛白杨与珠美海棠试管苗低温贮存技术初探

• 作 者: 白淑霞 刘立秋 陈文龙
• 机 构: 中国科学院石家庄农业现代化研究所,中国科学院石家庄农业现代化研究所,中国科学院石家庄农业现代化研究所 石家庄 050021,石家庄 050021,石家庄 050021
• 出 处: 生态农业研究
• 发表日期: 1996年04期
• 关键词: 试管苗 低温贮存 移栽成活率 生根苗 生理反应 抗冷性 培苗 培养基成分 继代 生理指标

林木试管苗通过0～10℃的自然低温贮存后,具有较高的成活率且抗冷性明显提高。观察和分析不同贮存阶段的试管苗形态和生理指标的变化,结果表明,试管苗随着贮存温度的降低,淀粉呈下降趋势,而可溶性糖相应上升,粗蛋白含量波动起伏略有升高,这是试管苗适应低温和弱光照的生理反应。贮存165d的分化苗和120d的生根苗污染损失率均低于10.0％;分化苗的有效苗数显著增加,生根率在95.5％以上,生根苗的移栽成活率为93.5％～98.5％。

Forest plantlets through the natural 0 ~ 10 ℃ low temperature storage, has a higher survival rate and significantly improved cold resistance. The morphological and physiological changes of in vitro seedlings were observed and analyzed. The results showed that with the decrease of storage temperature, the starch content of the seedlings decreased, but the soluble sugar increased accordingly, and the crude protein content increased slightly It is a physiological response that test-tube plantlets adapt to low temperature and weak light. The contamination loss rates of 165 days differentiated seedlings and 120 days of rooted shoots were less than 10.0%. The effective seedling number of differentiated seedlings increased significantly, the rooting rate was above 95.5%, and the survival rate of rooting seedlings was 93.5% -98.5%.