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采用荧光光谱法研究了羟基葫芦[6]脲(HOCB[6])对孟加拉红(TSS)的包结作用,考察了HOCB[6]浓度、缓冲液pH、温度、包结时间、有机溶剂等因素对包结作用的影响,结果表明,体系的荧光强度随着HOCB[6]浓度的升高而增强,呈现显著荧光增敏现象,同时荧光峰位有一定蓝移,Hildebrand-Benesi法计算结果显示HOCB[6]与TSS形成了1∶1的包结配合物,包结反应的热力学参数表明该包结过程为自发放热过程,这可能是主客体分子之间的疏水作用与离子偶极作用所引起的。
The effect of HOCB [6] on the entrapment of bengal red (TSS) was studied by fluorescence spectroscopy. The effects of HOCB [6], buffer pH, temperature, entrapment time, organic solvents and the like The results showed that the fluorescence intensity of the system increased with increasing concentration of HOCB [6], showing a significant fluorescence sensitization phenomenon. At the same time, there was a certain blue-shift of the fluorescence peak, and the calculated result of Hildebrand-Benesi method It is shown that HOCB [6] forms a 1: 1 inclusion complex with TSS. The thermodynamic parameters of the inclusion reaction indicate that the inclusion process is a spontaneous exothermic process, which may be due to the hydrophobic interaction between host and guest molecules and ionic dipole Caused by