论文部分内容阅读
为探讨茶碱对神经元凋亡的影响及其作用机理，建立小脑颗粒神经元的低钾凋亡模型，用ＤＮＡ凝胶电泳、ＦＤＡ染色和放射免疫等方法，发现经不同浓度茶碱（１～２０ｍｍｏｌ／Ｌ）处理，低钾培养的小脑颗粒神经元存活率相应提高２２％～１００％，量效关系有高度显著性（Ｐ＜０．０１），ＥＣ５０＝２．９７ｍｍｏｌ／Ｌ；经茶碱（２０ｍｍｏｌ／Ｌ）处理的细胞，其ＤＮＡ凝胶电泳带没有“梯子”状分布；与不同浓度茶碱（５～８０ｍｍｏｌ／Ｌ）在ＣＯ２培养箱孵育１５ｍｉｎ，小脑颗粒神经元内ｃＡＭＰ均升高，且是剂量依赖性的。认为茶碱对撤去极化浓度氯化钾诱发小脑颗粒神经元的凋亡有保护作用，此作用产生除与其升高小脑颗粒神经元内ｃＡＭＰ有关外，还有其它机理。
In order to explore the effect of theophylline on neuronal apoptosis and its mechanism of action, a model of cerebellar granule neurons of hypokalemia was established. By using DNA gel electrophoresis, FDA staining and radioimmunoassay, ~ 20mmol / L), the survival rate of cerebellar granule neurons cultured with low potassium increased by 22% -100%, the dose-response relationship was highly significant (P <0.01), EC50 = 2.97mmol / L; (20 mmol / L), there was no “ladder” distribution in the DNA gel electrophoresis bands. After incubated with different concentrations of theophylline (5 ~ 80 mmol / L) in CO2 incubator for 15 min, the cAMP in cerebellar granule neurons increased High, and dose-dependent. It is considered that theophylline has the protective effect on the apoptosis of cerebellar granule neurons evacuated by potassium chloride with the concentration of potassium chloride, and other mechanisms besides the increase of cAMP in cerebellar granule neurons.