恒河猴异体椎间盘移植组织学活性和生物化学研究

• 作 者: 周勇 阮狄克 陆瓞骥
• 机 构: TangDu Hospital, Fourth Military Medical University, Xi’an 710038,ChinaGeneral Hospital of Navy,Beij
• 出 处: 中国临床康复
• 发表日期: 2002年6期
• 关键词: intervertibral disc allograft uronic acid hydroxyproline proteoglycan collagen 3

Objective To investigate the histological viability and the biochemical change of fresh allografted intervertebral disc of rhesus monkey.Methods Under general anaesthesia,osteotomies of 12 rhesus monkeys were made at the endplates about 2.0 mm away from the L4～ 5 intervertibral disc,then the fresh discs were rntransposed in two matched monkeys. The four groups of animals were sampled after the third, sixth,ninth and twelfth month respectively.Histological viability was assessed by 3H Proline incorporation and biochemical changes were measured by water,proteoglycan and collagen content. Results Study of histological viability demonstrated that the DPM (Disintegration Per Minute) of 3H Proline increased at the third month in anulus fibrous and the sixth month in nucleous pulposus.In the nucleus pulposus of implanted disc the study of biochemistry showed that proteoglycan content was not change obviously nine months ago, but proteoglycan content decreased at the twelfth month.Collagen contents increased at the third,sixth and ninth month. Water contents were not change among twelve months. In the annulus fibrous of implanted disc,proteoglycan and water contents decreased at the ninth month, but they were increased at the twelfth month. Collagen content increased at the sixth month,but it decreased at the ninth month again. Conclusions The present results showed the intervertibral disc grafted survived allologous transplantation.The fresh allografted intervertibral disc appeared some ability of regeneration.rn