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目的建立测定石柱参药材及其片剂中5种人参皂苷含量的方法,为石柱参的质量控制提供科学依据。方法色谱柱为Kromasil C18柱;流动相为乙腈-水,梯度洗脱;柱温为25℃;流速为1.0 mL.min-1;检测波长为203 nm。结果5种人参皂苷的色谱峰与相邻色谱峰分离良好。人参皂苷Rg1质量浓度在19.8~198 mg.L-1内、人参皂苷Re质量浓度在20.6~206 mg.L-1内、人参皂苷Rb1质量浓度在33.0~330 mg.L-1内、人参皂苷Rc质量浓度在18.0~180 mg.L-1内、人参皂苷Rb2质量浓度在13.0~130 mg.L-1内与峰面积呈良好的线性关系。石柱参药材中人参皂苷Rg1、Re、Rb1、Rc、Rb2的平均回收率分别为99.8%、98.3%、99.2%、95.4%、96.8%,RSD分别为3.0%、3.0%、2.6%、2.2%、2.8%(n=6);石柱参片剂中人参皂苷Rg1、Re、Rb1、Rc、Rb2的平均回收率分别为100.2%、99.0%、99.7%、96.4%、98.4%,RSD分别为2.2%、3.1%、3.6%、2.6%、2.8%(n=6)。结论本实验中所建立的方法可用于石柱参药材及其片剂的质量控制。
OBJECTIVE To establish a method for the determination of five kinds of ginsenosides in Radix Ginseng and its tablets and provide a scientific basis for the quality control of Radix Ginseng. Methods The column was Kromasil C18. The mobile phase consisted of acetonitrile-water with gradient elution. The column temperature was 25 ℃ and the flow rate was 1.0 mL · min-1. The detection wavelength was 203 nm. Results The chromatograms of the five ginsenosides were separated from the adjacent peaks. The concentration of ginsenoside Rg1 was 19.8-198 mg.L-1, the concentration of ginsenoside Re was 20.6-206 mg.L-1, the concentration of ginsenoside Rb1 was 33.0-330 mg.L-1, the concentration of ginsenoside Rc mass concentration ranged from 18.0 to 180 mg.L-1, ginsenoside Rb2 mass concentration ranged from 13.0 to 130 mg.L-1 and peak area showed a good linear relationship. The average recoveries of ginsenosides Rg1, Re, Rb1, Rc and Rb2 in Radix Ginseng were 99.8%, 98.3%, 99.2%, 95.4% and 96.8%, respectively, and the RSDs were 3.0%, 3.0%, 2.6% and 2.2% , 2.8% (n = 6) respectively. The average recoveries of ginsenosides Rg1, Re, Rb1, Rc and Rb2 in Shizhu Ginseng Tablet were 100.2%, 99.0%, 99.7%, 96.4% and 98.4% %, 3.1%, 3.6%, 2.6%, 2.8% (n = 6). Conclusion The method established in this experiment can be used for the quality control of Radix Ginseng and its tablets.