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本文旨在研究豚鼠经皮下注射新霉素后内耳组织及其它器官中的药物浓度。实验动物按100mg/kg/日给药,用药时间分别为1、3、6天和1、2、3周,均于末次注射后24小时杀死。引流内耳淋巴液后,内耳组织作“干”性解剖,即不浸入生理盐水,将分离的螺旋器和侧壁组织(血管纹和螺旋韧带)置于0.2M磷酸钠(pH8)中捣匀,然后离心取其上层清液,按Davis(Appl Microbiol 22∶659,1971)介绍的抗生素微量生物测定法测定其中新霉素的含量;并以同法测定豚鼠心、肝、肺、脾、肾和脑组织中的含量。
This article aims to investigate the drug concentration in the inner ear tissue and other organs after guinea pigs have been injected neomycin subcutaneously. The experimental animals were dosed at 100 mg / kg / day for 1, 3, 6 days and 1, 2, and 3 weeks respectively, all of which were killed 24 hours after the last injection. Drainage of the inner ear lymph, the inner ear tissue for “dry” anatomy, that is not immersed in saline, the separation of the spiral and the side wall tissue (vascular pattern and spiral ligament) placed 0.2M sodium phosphate (pH8) The supernatant was then centrifuged and the content of neomycin was determined by the microbiological assay of antibiotics introduced by Davis (Appl Microbiol 22:659, 1971). The contents of neomycin in guinea pig heart, liver, lung, spleen, kidney and Content in brain tissue.