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目的研究乙肝病毒携带者外周血HBV-DNA阳性与羊水HBV-DNA阳性的关系,以及是否经过胎盘穿刺对羊水HBV-DNA拷贝数的影响。方法 2011年10月~2012年10月有产前诊断指征需要进行羊水穿刺的乙肝病毒携带者孕妇,在离心取细胞沉淀培养后的羊水上清,测量羊水上清的HBV-DNA拷贝数;并与其外周血乙肝DNA拷贝数做统计学对比。以及比较是否经过胎盘对羊水乙肝DNA拷贝数的影响。结果外周血HBV-DNA阳性的孕妇,其羊水HBV-DNA阳性率明显高于外周血HBV-DNA阴性的孕妇。结论经过胎盘穿刺对羊水HBV-DNA阳性率的影响无统计学意义。
Objective To study the relationship between the positive HBV-DNA in peripheral blood of HBV carriers and HBV-DNA in amniotic fluid and the influence of placenta puncture on the HBV-DNA copy number in amniotic fluid. Methods From October 2011 to October 2012, pregnant women with hepatitis B virus carriers requiring amniocentesis for prenatal diagnosis were enrolled. After centrifugation, the amniotic fluid supernatant was cultured and the copy number of HBV-DNA in amniotic fluid supernatant was measured. And compared with the peripheral blood HBV DNA copy number do statistics. And compare whether the placenta on amniotic fluid HBV DNA copy number. Results The positive rate of HBV-DNA in amniotic fluid of HBV-DNA positive pregnant women was significantly higher than that of HBV-DNA negative pregnant women in peripheral blood. Conclusion The positive rate of HBV-DNA in amniotic fluid after placental puncture is not statistically significant.